CXCL16 Antibody (C-5): sc-514363

CXCL16 Antibody (C-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects CXCL16 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-CXCL16 antibody (C-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CXCL16, also known as SR-PSOX (scavenger receptor for phosphatidylserine and oxidized lipoprotein), plays a crucial role in immune response by binding to the CXCR6 receptor, which is essential for the chemotactic migration of immune cells to sites of inflammation. This interaction not only induces a strong chemotactic response but also triggers calcium mobilization, highlighting CXCL16′s importance in regulating immune cell trafficking and activation. Structurally, CXCL16 is classified as an alpha (CXC) chemokine, exhibiting features of CC chemokines and sharing a similar structure to fractalkine, characterized by a transmembrane region and a chemokine domain connected by a mucin-like stalk. Northern blot analysis has revealed the presence of a 2.2-kb CXCL16 transcript in various human tissues, including the spleen, lymph nodes, Peyer patches, lung, kidney, small intestine, and thymus, with minimal expression in the heart and liver, and absent in the brain and bone marrow. Flow cytometry (FCM) and western blotting (WB) further confirm the expression of a glycosylated cell-surface protein and a soluble protein in cell supernatants, underscoring CXCL16′s diverse functional roles in both cellular and soluble forms.

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CXCL16 Antibody (C-5) References:

1. A transmembrane CXC chemokine is a ligand for HIV-coreceptor Bonzo.  |  Matloubian, M., et al. 2000. Nat Immunol. 1: 298-304. PMID: 11017100
2. Expression cloning of the STRL33/BONZO/TYMSTRligand reveals elements of CC, CXC, and CX3C chemokines.  |  Wilbanks, A., et al. 2001. J Immunol. 166: 5145-54. PMID: 11290797
3. [New oxidized LDL receptors and their functions in atherogenesis].  |  Kume, N. 2002. Nihon Ronen Igakkai Zasshi. 39: 264-7. PMID: 12073583
4. Expression of CXCL16 in human T cells.  |  Shashkin, P., et al. 2003. Arterioscler Thromb Vasc Biol. 23: 148-9. PMID: 12524239
5. Cutting edge: profile of chemokine receptor expression on human plasma cells accounts for their efficient recruitment to target tissues.  |  Nakayama, T., et al. 2003. J Immunol. 170: 1136-40. PMID: 12538668
6. CXCL16 signals via Gi, phosphatidylinositol 3-kinase, Akt, I kappa B kinase, and nuclear factor-kappa B and induces cell-cell adhesion and aortic smooth muscle cell proliferation.  |  Chandrasekar, B., et al. 2004. J Biol Chem. 279: 3188-96. PMID: 14625285
7. Upregulation of SR-PSOX/CXCL16 and recruitment of CD8+ T cells in cardiac valves during inflammatory valvular heart disease.  |  Yamauchi, R., et al. 2004. Arterioscler Thromb Vasc Biol. 24: 282-7. PMID: 14699018
8. CXCL16/SR-PSOX is an interferon-gamma-regulated chemokine and scavenger receptor expressed in atherosclerotic lesions.  |  Wuttge, DM., et al. 2004. Arterioscler Thromb Vasc Biol. 24: 750-5. PMID: 14988089
9. CXCR6/CXCL16 functions as a regulator in metastasis and progression of cancer.  |  Deng, L., et al. 2010. Biochim Biophys Acta. 1806: 42-9. PMID: 20122997
10. Exosomes derived from Fusobacterium nucleatum-infected colorectal cancer cells facilitate tumour metastasis by selectively carrying miR-1246/92b-3p/27a-3p and CXCL16.  |  Guo, S., et al. 2020. Gut.. PMID: 33172926
11. CXCL16 inhibits epithelial regeneration and promotes fibrosis during the progression of radiation enteritis.  |  Cui, Y., et al. 2023. J Pathol. 259: 180-193. PMID: 36373877
12. TLR7-MyD88-DC-CXCL16 axis results neutrophil activation to elicit inflammatory response in pustular psoriasis.  |  Lu, J., et al. 2023. Cell Death Dis. 14: 315. PMID: 37160878

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CXCL16 Antibody (C-5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-CXCL16 Antibody (C-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CXCL16 Antibody (C-5) Specifications

Alternate Product Name: CXCL16

Product Clone Name: C-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 117-148

Epitope Target: CXCL16

Epitope Species: human

Product Reactivity/Detection: CXCL16

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for CXCL16 Antibody (C-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin