CXCL16 CRISPR/Cas9 KO Plasmid (m): sc-425819
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CXCL16 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CXCL16 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CXCL16 CRISPR/Cas9 KO Plasmid (m) | sc-425819 | 20 µg | $397.00 |
Overview
Cxcl16 encodes CXCL16, a transmembrane chemokine that can be proteolytically shed to generate a soluble ligand for CXCR6, coordinating chemotactic recruitment and retention of activated T cells, NK T cells, and other leukocyte subsets. CXCL16 participates in inflammatory signaling networks that shape tissue immune surveillance, leukocyte adhesion, and cytokine-driven crosstalk within the vascular and parenchymal microenvironments. In mouse models, CXCL16/CXCR6 signaling has been linked to processes such as atherosclerotic inflammation, liver and lung immune cell trafficking, and tumor–immune interactions, making it relevant to studies of chronic inflammation and immune-mediated pathology. Its regulation intersects with NF-κB–associated inflammatory programs and ectodomain shedding pathways that control the balance of membrane-bound versus soluble chemokine activity.
CXCL16 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cxcl16 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cxcl16 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cxcl16 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CXCL16 protein expression.
This CRISPR knockout system enables efficient generation of Cxcl16-deficient cell models for investigation of CXCL16 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cxcl16 exon(s) critical for CXCL16 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cxcl16 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CXCL16 CRISPR/Cas9 KO Plasmid (m) and CXCL16 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cxcl16 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CXCL16 HDR Plasmid (m) and CXCL16 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cxcl16 homology arms to support homology-directed repair at defined Cxcl16 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.