CUL-1 Antibody (19) recommended for detection of CUL-1 of mouse, rat, human and canine origin by WB and IP

CUL-1 Antibody (19): sc-135874

CUL-1 Antibody (19) is rated 4.0 out of 5 by 1.
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Datasheets

    • CUL-1 Antibody (19) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
    • raised against amino acids 56-246 of CUL-1 of mouse origin
    • recommended for detection of CUL-1 of mouse, rat, human and canine origin by WB and IP
    • See CUL-1 (D-5): sc-17775 for CUL-1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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I am using CUL-1 (19): sc-135874 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Professor Griffin
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Robust signal in WB in P19 whole cell lysate Robust signal in WB in P19 whole cell lysate. -SCBT QC
Date published: 2013-11-23
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