CTPS1 Antibody (2G7-1D10): sc-293266

CTPS1 Antibody (2G7-1D10) is a mouse monoclonal IgG2b kappa light chain antibody that detects CTPS1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-CTPS1 antibody (2G7-1D10) is available as the non-conjugated format. CTPS1, also known as cytidine-5-prime-triphosphate synthase, is a 591 amino acid enzyme that plays a pivotal role in pyrimidine metabolism, specifically catalyzing the ATP-dependent conversion of uridine triphosphate (UTP) to cytidine triphosphate (CTP). This reaction is a rate-limiting step in the synthesis of nucleic acids, making CTPS1 essential for proper cell growth and development. CTPS1 is primarily located in the cytoplasm, where CTPS1 facilitates the availability of CTP, a critical building block for RNA synthesis and cellular energy metabolism. CTPS1 regulation is complex, involving various post-translational modifications, including phosphorylation by protein kinase C, which can modulate CTPS1 activity and influence cellular responses to growth signals. Notably, the gene encoding CTPS1 is situated on chromosome 1, a region frequently implicated in the progression of several cancers, highlighting CTPS1′s potential involvement in tumorigenesis and making CTPS1 a significant target for cancer research.

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CTPS1 Antibody (2G7-1D10) References:

1. Cytidine triphosphate synthase activity and mRNA expression in normal human blood cells.  |  Verschuur, AC., et al. 1999. Biol Chem. 380: 41-6. PMID: 10064135
2. Crystal structures of CTP synthetase reveal ATP, UTP, and glutamine binding sites.  |  Goto, M., et al. 2004. Structure. 12: 1413-23. PMID: 15296735
3. Expression of Human CTP synthetase in Saccharomyces cerevisiae reveals phosphorylation by protein kinase A.  |  Han, GS., et al. 2005. J Biol Chem. 280: 38328-36. PMID: 16179339
4. Mechanisms of product feedback regulation and drug resistance in cytidine triphosphate synthetases from the structure of a CTP-inhibited complex.  |  Endrizzi, JA., et al. 2005. Biochemistry. 44: 13491-9. PMID: 16216072
5. Structure of the synthetase domain of human CTP synthetase, a target for anticancer therapy.  |  Kursula, P., et al. 2006. Acta Crystallogr Sect F Struct Biol Cryst Commun. 62: 613-7. PMID: 16820675
6. Phosphorylation of human CTP synthetase 1 by protein kinase C: identification of Ser(462) and Thr(455) as major sites of phosphorylation.  |  Chang, YF., et al. 2007. J Biol Chem. 282: 17613-22. PMID: 17463002
7. Specific reactions of S-nitrosothiols with cysteine hydrolases: A comparative study between dimethylargininase-1 and CTP synthetase.  |  Braun, O., et al. 2007. Protein Sci. 16: 1522-34. PMID: 17600152
8. Regulation of human cytidine triphosphate synthetase 1 by glycogen synthase kinase 3.  |  Higgins, MJ., et al. 2007. J Biol Chem. 282: 29493-503. PMID: 17681942
9. CTPS1 promotes malignant progression of triple-negative breast cancer with transcriptional activation by YBX1.  |  Lin, Y., et al. 2022. J Transl Med. 20: 17. PMID: 34991621
10. CTPS1 inhibition suppresses proliferation and migration in colorectal cancer cells.  |  Wu, F., et al. 2022. Cell Cycle. 21: 2563-2574. PMID: 35912542
11. Selective pharmacologic targeting of CTPS1 shows single-agent activity and synergizes with BCL2 inhibition in aggressive mantle cell lymphoma.  |  Durand, R., et al. 2024. Haematologica. 109: 2574-2584. PMID: 38385294
12. Clustered base substitutions in CTP synthetase conferring drug resistance in Chinese hamster ovary cells.  |  Whelan, J., et al. 1993. Nat Genet. 3: 317-22. PMID: 7981751