CTMP CRISPR/Cas9 KO Plasmid (m): sc-429205
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CTMP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CTMP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CTMP CRISPR/Cas9 KO Plasmid (m) | sc-429205 | 20 µg | $397.00 |
Overview
Them4 encodes CTMP (carboxyl-terminal modulator protein), a membrane-associated regulator best known for modulating AKT/PKB signaling. CTMP can bind AKT and influence its phosphorylation state, linking Them4 to PI3K–AKT pathway control of cell survival, metabolism, and stress responses. In mouse systems, altered CTMP activity has been associated with changes in mitochondrial function, oxidative stress handling, and apoptosis-related processes. Because PI3K–AKT signaling is broadly implicated in inflammatory, metabolic, and neurodegenerative phenotypes, Them4 is frequently studied as a node connecting signaling dynamics to disease-relevant cellular states.
CTMP CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Them4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Them4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Them4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CTMP protein expression.
This CRISPR knockout system enables efficient generation of Them4-deficient cell models for investigation of CTMP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Them4 exon(s) critical for CTMP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Them4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CTMP CRISPR/Cas9 KO Plasmid (m) and CTMP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Them4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CTMP HDR Plasmid (m) and CTMP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Them4 homology arms to support homology-directed repair at defined Them4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.