Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CTL2 Double Nickase Plasmid (h): sc-402652-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CTL2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CTL2 Double Nickase Plasmid (h) and CTL2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC44A2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CTL2 Antibody (F7): sc-101266
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CTL2 Double Nickase Plasmid (h) | sc-402652-NIC | 20 µg | $410.00 | |||
CTL2 Double Nickase Plasmid (h2) | sc-402652-NIC-2 | 20 µg | $410.00 |
SLC44A2 encodes choline transporter-like protein 2 (CTL2), a multi-pass membrane protein implicated in choline transport and phospholipid metabolism that supports membrane biogenesis and cellular homeostasis. CTL2 activity interfaces with pathways requiring choline-derived phosphatidylcholine, linking it to organelle function, cell signaling, and stress responses. In hematopoietic and vascular contexts, SLC44A2 has been associated with platelet and neutrophil biology and thrombo-inflammatory processes, and it is also recognized as the HNA-3 antigen relevant to immune-mediated reactions. Altered SLC44A2 regulation has been explored in settings involving inflammation, vascular disease mechanisms, and tissue-specific membrane lipid remodeling.
CTL2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC44A2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC44A2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC44A2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC44A2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.