CTGF (h): 293T Lysate: sc-111612

CTGF (h): 293T Lysate is derived from the 293T cell line, a human embryonic kidney cell line modified to express the SV40 large T antigen, enhancing its transfection efficiency and protein production capabilities. This lysate is specifically enriched with human Connective Tissue Growth Factor (CTGF), a secreted protein that plays a critical role in cell adhesion, migration, proliferation, and extracellular matrix (ECM) production. CTGF is involved in various signaling pathways and interacts with multiple receptors, including integrins and growth factor receptors, mediating cellular responses to tissue injury and remodeling. In research, CTGF (h): 293T Lysate is used to study the molecular mechanisms of CTGF's action and its role in cellular and extracellular processes. Researchers employ this lysate in Western blotting to detect and quantify CTGF expression levels under different experimental conditions, providing insights into its regulation and involvement in ECM production. The lysate is also utilized in co-immunoprecipitation assays to identify protein-protein interactions involving CTGF, helping to explain its role in signaling networks and its interactions with other ECM components and receptors. Functional assays using CTGF (h): 293T Lysate can assess the impact of CTGF on cell behavior, such as promoting cell adhesion, migration, and proliferation. These studies contribute to understanding how CTGF influences cellular responses to injury and remodeling, particularly in the context of fibrosis and tissue repair.