CTDSP1 CRISPR/Cas9 KO Plasmid (h): sc-406965
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CTDSP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CTDSP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CTDSP1 CRISPR/Cas9 KO Plasmid (h) | sc-406965 | 20 µg | $397.00 |
Overview
CTDSP1 (CTD small phosphatase 1) is a nuclear haloacid dehalogenase (HAD)-family phosphatase that dephosphorylates the C-terminal domain of RNA polymerase II, contributing to transcription cycle control and coordination of gene expression programs. By modulating phosphorylation states that influence promoter clearance and transcriptional elongation, CTDSP1 helps shape RNA processing and broader transcriptional homeostasis. CTDSP1 has also been linked to regulation of signaling and differentiation-associated transcriptional networks, making it relevant to studies of cell state transitions and stress responses. Dysregulated CTDSP1 activity or expression has been investigated in the context of altered transcriptional regulation observed across multiple disease models, supporting its utility as a mechanistic target in functional genomics.
CTDSP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CTDSP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CTDSP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CTDSP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CTDSP1 protein expression.
This CRISPR knockout system enables efficient generation of CTDSP1-deficient cell models for investigation of CTDSP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CTDSP1 exon(s) critical for CTDSP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CTDSP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CTDSP1 CRISPR/Cas9 KO Plasmid (h) and CTDSP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CTDSP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CTDSP1 HDR Plasmid (h) and CTDSP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CTDSP1 homology arms to support homology-directed repair at defined CTDSP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.