CstF-77 Antibody (G-5): sc-376575
- CstF-77 Antibody (G-5) is a mouse monoclonal IgG2a κ CstF-77 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 31-330 mapping near the N-terminus of CstF-77 of human origin
- CstF-77 Antibody (G-5) is recommended for detection of CstF-77 of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and avian
- Anti-CstF-77 Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CstF-77 Antibody (G-5) for WB applications. These reagents are now offered in bundles with CstF-77 Antibody (G-5) (see ordering information below).
CstF-77 Antibody (G-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects CstF-77 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CstF-77 antibody (G-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CstF-77, also known as cleavage stimulation factor 77 kDa subunit or CstF3, plays a crucial role in mRNA precursor polyadenylation, a vital process for gene expression regulation. CstF-77 functions as part of a heterotrimeric complex called CstF, which recognizes specific RNA sequences downstream of the polyadenylation site. CstF-77 bridges CstF-64 and CstF-50 subunits while featuring an α-helical structure with 11 Half-a-TPR-containing repeats, essential for stability and function. CstF-77 also interacts with key polyadenylation proteins like CPSF1 and FCP1, highlighting an integral role in mRNA processing and gene expression regulation across eukaryotic organisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CstF-77 Antibody (G-5) References:
- Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50. | Kleiman, FE. and Manley, JL. 1999. Science. 285: 1576-9. PMID: 10477523
- Complex protein interactions within the human polyadenylation machinery identify a novel component. | Takagaki, Y. and Manley, JL. 2000. Mol Cell Biol. 20: 1515-25. PMID: 10669729
- Chimeric human CstF-77/Drosophila Suppressor of forked proteins rescue suppressor of forked mutant lethality and mRNA 3' end processing in Drosophila. | Benoit, B., et al. 2002. Proc Natl Acad Sci U S A. 99: 10593-8. PMID: 12149458
- An intronic polyadenylation site in human and mouse CstF-77 genes suggests an evolutionarily conserved regulatory mechanism. | Pan, Z., et al. 2006. Gene. 366: 325-34. PMID: 16316725
- Crystal structure of murine CstF-77: dimeric association and implications for polyadenylation of mRNA precursors. | Bai, Y., et al. 2007. Mol Cell. 25: 863-75. PMID: 17386263
- The structure of the CstF-77 homodimer provides insights into CstF assembly. | Legrand, P., et al. 2007. Nucleic Acids Res. 35: 4515-22. PMID: 17584787
- A multisubunit factor, CstF, is required for polyadenylation of mammalian pre-mRNAs. | Takagaki, Y., et al. 1990. Genes Dev. 4: 2112-20. PMID: 1980119
- RNA recognition by the human polyadenylation factor CstF. | Takagaki, Y. and Manley, JL. 1997. Mol Cell Biol. 17: 3907-14. PMID: 9199325
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CstF-77 Antibody (G-5) | sc-376575 | 200 µg/ml | $322.00 | |||
CstF-77 Antibody (G-5): m-IgGκ BP-HRP Bundle | sc-523015 | 200 µg Ab, 40 µg BP | $361.00 | |||
CstF-77 Antibody (G-5): m-IgG2a BP-HRP Bundle | sc-546132 | 200 µg Ab; 10 µg BP | $361.00 | |||
CstF-77 Antibody (G-5) AC | sc-376575 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CstF-77 Antibody (G-5) HRP | sc-376575 HRP | 200 µg/ml | $322.00 | |||
CstF-77 Antibody (G-5) FITC | sc-376575 FITC | 200 µg/ml | $336.00 | |||
CstF-77 Antibody (G-5) PE | sc-376575 PE | 200 µg/ml | $349.00 | |||
CstF-77 Antibody (G-5) Alexa Fluor® 488 | sc-376575 AF488 | 200 µg/ml | $364.00 | |||
CstF-77 Antibody (G-5) Alexa Fluor® 546 | sc-376575 AF546 | 200 µg/ml | $364.00 | |||
CstF-77 Antibody (G-5) Alexa Fluor® 594 | sc-376575 AF594 | 200 µg/ml | $364.00 | |||
CstF-77 Antibody (G-5) Alexa Fluor® 647 | sc-376575 AF647 | 200 µg/ml | $364.00 | |||
CstF-77 Antibody (G-5) Alexa Fluor® 680 | sc-376575 AF680 | 200 µg/ml | $364.00 | |||
CstF-77 Antibody (G-5) Alexa Fluor® 790 | sc-376575 AF790 | 200 µg/ml | $364.00 |