Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CSN8 Double Nickase Plasmid (m): sc-430844-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CSN8 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CSN8 Double Nickase Plasmid (m) and CSN8 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Cops8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CSN8 Antibody (C-9): sc-514088
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CSN8 Double Nickase Plasmid (m) | sc-430844-NIC | 20 µg | $410.00 |
Cops8 encodes CSN8, a core subunit of the COP9 signalosome that regulates cullin–RING ubiquitin ligase activity through deneddylation, thereby shaping ubiquitin-dependent protein turnover. By controlling the stability of cell-cycle regulators, DNA damage response factors, and signaling intermediates, CSN8 helps coordinate proteostasis, stress responses, and transcriptional programs. In mouse systems, disruption of COP9 signalosome function has been linked to altered developmental homeostasis and tissue-specific defects, reflecting broad impacts on proliferation and apoptosis. These roles make Cops8 a useful node for studying ubiquitin–proteasome pathway crosstalk with signaling networks such as MAPK and NF-κB.
CSN8 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Cops8 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Cops8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Cops8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Cops8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.