Csk Double Nickase Plasmid (h): sc-400992-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Csk Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Csk Double Nickase Plasmid (h) and Csk Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CSK. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Csk Antibody (E-3): sc-166560
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Csk Double Nickase Plasmid (h) | sc-400992-NIC | 20 µg | $410.00 | |||
Csk Double Nickase Plasmid (h2) | sc-400992-NIC-2 | 20 µg | $410.00 |
CSK encodes Csk, a cytosolic tyrosine kinase that negatively regulates Src-family kinases by phosphorylating their C-terminal inhibitory tyrosine, thereby constraining signaling amplitude downstream of immune receptors, integrins, and growth factor receptors. Through this gatekeeping function, Csk influences cell adhesion and migration, cytoskeletal remodeling, and receptor-proximal signal transduction in pathways such as T cell receptor signaling and focal adhesion signaling. Altered CSK activity or regulation can perturb phosphorylation networks that shape immune cell activation thresholds and proliferative signaling programs. Dysregulated Src-family kinase control linked to CSK has been associated with immune-mediated and oncogenic signaling contexts, supporting its study in mechanistic models of signaling homeostasis.
Csk Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CSK locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CSK. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CSK function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CSK-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.