CSH1 CRISPR/Cas9 KO Plasmid (h): sc-401213
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CSH1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CSH1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CSH1 CRISPR/Cas9 KO Plasmid (h) | sc-401213 | 20 µg | $397.00 |
Overview
CSH1 encodes chorionic somatomammotropin hormone 1, a placenta-derived member of the growth hormone/prolactin family that contributes to endocrine regulation during pregnancy. CSH1 signaling engages cytokine receptor–associated pathways, including JAK/STAT and downstream transcriptional programs that influence trophoblast function, nutrient partitioning, and maternal metabolic adaptation. Altered placental expression of CSH1 and related lactogenic hormones has been investigated in the context of impaired placentation and dysregulated fetal growth phenotypes. As a result, CSH1 is commonly studied in trophoblast biology, placental development, and endocrine signaling networks that coordinate maternal–fetal interactions.
CSH1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CSH1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CSH1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CSH1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CSH1 protein expression.
This CRISPR knockout system enables efficient generation of CSH1-deficient cell models for investigation of CSH1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CSH1 exon(s) critical for CSH1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CSH1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CSH1 CRISPR/Cas9 KO Plasmid (h) and CSH1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CSH1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CSH1 HDR Plasmid (h) and CSH1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CSH1 homology arms to support homology-directed repair at defined CSH1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.