Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CSB Double Nickase Plasmid (h): sc-401287-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CSB Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CSB Double Nickase Plasmid (h) and CSB Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ERCC6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CSB Antibody (D-7): sc-166042
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CSB Double Nickase Plasmid (h) | sc-401287-NIC | 20 µg | $410.00 | |||
CSB Double Nickase Plasmid (h2) | sc-401287-NIC-2 | 20 µg | $410.00 |
ERCC6 encodes the human Cockayne syndrome B protein (CSB), an ATP-dependent SWI2/SNF2-family DNA helicase–like factor that couples transcription to nucleotide excision repair. CSB is central to transcription-coupled NER by sensing RNA polymerase II–stalled lesions, coordinating chromatin remodeling, and recruiting repair machinery to restore transcriptional elongation. Beyond TC-NER, CSB contributes to genome stability through roles in oxidative DNA damage responses, replication stress management, and mitochondrial homeostasis. Disruption of ERCC6 function is linked to Cockayne syndrome and related neurodevelopmental and progeroid phenotypes, making CSB a key node for studying DNA damage signaling and transcription-associated repair defects.
CSB Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ERCC6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ERCC6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ERCC6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ERCC6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.