CSB Antibody (D-7): sc-166042

CSB Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects CSB protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-CSB antibody (D-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CSB protein, also known as excision repair cross-complementing protein-6 (ERCC-6), plays a crucial role in the nucleotide excision repair pathway, specifically in the repair of DNA lesions that occur on the transcribed strand of active genes. This preferential repair mechanism, known as transcription-coupled repair, is vital for maintaining genomic stability and preventing mutations that can lead to diseases such as Cockayne syndrome, a disorder characterized by sensitivity to UV light and developmental defects. Mutations in CSB gene disrupt this repair process, leading to the accumulation of DNA damage in actively transcribed regions, which underscores CSB′s importance in cellular response to DNA damage and overall cellular health.

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CSB Antibody (D-7) References:

1. ERCC6, a member of a subfamily of putative helicases, is involved in Cockayne's syndrome and preferential repair of active genes.  |  Troelstra, C., et al. 1992. Cell. 71: 939-53. PMID: 1339317
2. The role of Cockayne Syndrome group B (CSB) protein in base excision repair and aging.  |  Stevnsner, T., et al. 2008. Mech Ageing Dev. 129: 441-8. PMID: 18541289
3. CSB cooperates with SMARCAL1 to maintain telomere stability in ALT cells.  |  Feng, E., et al. 2020. J Cell Sci. 133: PMID: 31974116
4. A CSB-PAF1C axis restores processive transcription elongation after DNA damage repair.  |  van den Heuvel, D., et al. 2021. Nat Commun. 12: 1342. PMID: 33637760
5. Functions of the CSB Protein at Topoisomerase 2 Inhibitors-Induced DNA Lesions.  |  Busatto, FF., et al. 2021. Front Cell Dev Biol. 9: 727836. PMID: 34746125
6. CSB Regulates Pathway Choice in Response to DNA Replication Stress Induced by Camptothecin.  |  Batenburg, NL., et al. 2023. Int J Mol Sci. 24: PMID: 37569794
7. The Cockayne syndrome group A gene encodes a WD repeat protein that interacts with CSB protein and a subunit of RNA polymerase II TFIIH.  |  Henning, KA., et al. 1995. Cell. 82: 555-64. PMID: 7664335
8. Structure and expression of the excision repair gene ERCC6, involved in the human disorder Cockayne's syndrome group B.  |  Troelstra, C., et al. 1993. Nucleic Acids Res. 21: 419-26. PMID: 8382798
9. Role of CSB/p38/RK stress response kinase in LPS and cytokine signaling mechanisms.  |  Lee, JC. and Young, PR. 1996. J Leukoc Biol. 59: 152-7. PMID: 8603987
10. Interactions involving the human RNA polymerase II transcription/nucleotide excision repair complex TFIIH, the nucleotide excision repair protein XPG, and Cockayne syndrome group B (CSB) protein.  |  Iyer, N., et al. 1996. Biochemistry. 35: 2157-67. PMID: 8652557
11. The Cockayne syndrome B protein, involved in transcription-coupled DNA repair, resides in an RNA polymerase II-containing complex.  |  van Gool, AJ., et al. 1997. EMBO J. 16: 5955-65. PMID: 9312053
12. RNA polymerase II elongation complexes containing the Cockayne syndrome group B protein interact with a molecular complex containing the transcription factor IIH components xeroderma pigmentosum B and p62.  |  Tantin, D. 1998. J Biol Chem. 273: 27794-9. PMID: 9774388

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CSB Antibody (D-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-CSB Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CSB Antibody (D-7) Specifications

Alternate Product Name: ERCC6

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: CSB

Epitope Species: human

Product Reactivity/Detection: CSB

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for CSB Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin