Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CS1 Double Nickase Plasmid (h): sc-401906-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CS1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CS1 Double Nickase Plasmid (h) and CS1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLAMF7. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CS1 Antibody (162.1): sc-53577
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CS1 Double Nickase Plasmid (h) | sc-401906-NIC | 20 µg | $410.00 | |||
CS1 Double Nickase Plasmid (h2) | sc-401906-NIC-2 | 20 µg | $410.00 |
SLAMF7, also known as CS1, is a human immunoreceptor expressed predominantly on natural killer cells, subsets of T cells, and plasma cells, where it mediates cell–cell recognition and immunomodulatory signaling. Through homotypic interactions and recruitment of adaptor proteins such as EAT-2, CS1 influences cytotoxic activation, cytokine release, and immune synapse formation. SLAMF7-dependent pathways intersect with regulation of lymphocyte activation and differentiation, shaping inflammatory and antitumor immune responses. Dysregulated SLAMF7 expression and signaling are frequently studied in the context of plasma cell biology, immune evasion, and hematologic disease mechanisms.
CS1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLAMF7 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLAMF7. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLAMF7 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLAMF7-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.