CRY2 Double Nickase Plasmid (h): sc-403171-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CRY2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CRY2 Double Nickase Plasmid (h) and CRY2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CRY2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CRY2 Antibody (3H4): sc-293263
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRY2 Double Nickase Plasmid (h) | sc-403171-NIC | 20 µg | $410.00 | |||
CRY2 Double Nickase Plasmid (h2) | sc-403171-NIC-2 | 20 µg | $410.00 |
CRY2 encodes cryptochrome 2, a flavoprotein component of the core circadian clock that functions primarily as a transcriptional repressor within the CLOCK–BMAL1 feedback loop. By partnering with PER proteins and modulating E-box–driven transcription, CRY2 helps coordinate daily oscillations in gene expression that impact cell cycle control, DNA damage responses, metabolism, and endocrine signaling. CRY2 activity is linked to ubiquitin-dependent protein turnover and phosphorylation pathways that tune circadian period and amplitude. Dysregulation of CRY2-associated circadian programs has been implicated in sleep and chronobiology phenotypes and is frequently studied in the context of metabolic and proliferative disorders where clock disruption alters cellular homeostasis.
CRY2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CRY2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CRY2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CRY2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CRY2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.