Crk II Antibody (2210C1a): sc-81234

Crk II Antibody (2210C1a) is a mouse monoclonal IgG1 antibody that detects Crk II in human samples through applications such as western blotting (WB) and immunoprecipitation (IP). Crk II, a member of the Crk family of adapter proteins, plays a crucial role in cellular signaling pathways by facilitating the interaction between various signaling molecules. Anti-Crk II antibody (2210C1a) targets a protein primarily located in the cytoplasm, where Crk II acts as a mediator of signal transduction, particularly in response to growth factors like insulin-like growth factor I (IGF-I). The localization of Crk II is significant because Crk II allows for rapid and efficient transmission of signals that regulate cell proliferation, differentiation, and survival. Notably, Crk II is rapidly tyrosine-phosphorylated upon IGF-I stimulation, indicating Crk II involvement in the IGF-I receptor signaling pathway, which is essential for normal cellular function and has implications in cancer biology. The gene encoding Crk I and II is situated on human chromosome 17p13, a region frequently associated with deletions and loss of heterozygosity in various cancers, highlighting the importance of Crk II in both normal physiology and disease states. Crk II monoclonal antibody (2210C1a) is an invaluable tool for researchers investigating the intricate signaling networks that govern cellular behavior and their dysregulation in cancer.

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Crk II Antibody (2210C1a) References:

1. Mutant mice lacking Crk-II caused by the gene trap insertional mutagenesis: Crk-II is not essential for embryonic development.  |  Imaizumi, T., et al. 1999. Biochem Biophys Res Commun. 266: 569-74. PMID: 10600543
2. Phosphorylation of c-Crk II on the negative regulatory Tyr222 mediates nerve growth factor-induced cell spreading and morphogenesis.  |  Escalante, M., et al. 2000. J Biol Chem. 275: 24787-97. PMID: 10825157
3. Activation of the focal adhesion kinase signaling pathway by structural alterations in the carboxyl-terminal region of c-Crk II.  |  Zvara, A., et al. 2001. Oncogene. 20: 951-61. PMID: 11314030
4. A Crk-II/TC10 signaling pathway is required for osmotic shock-stimulated glucose transport.  |  Gual, P., et al. 2002. J Biol Chem. 277: 43980-6. PMID: 12215429
5. Transactivation of Abl by the Crk II adapter protein requires a PNAY sequence in the Crk C-terminal SH3 domain.  |  Reichman, C., et al. 2005. Oncogene. 24: 8187-99. PMID: 16158059
6. Proapoptotic function of the nuclear Crk II adaptor protein.  |  Kar, B., et al. 2007. Biochemistry. 46: 10828-40. PMID: 17764157
7. PAK1 kinase promotes cell motility and invasiveness through CRK-II serine phosphorylation in non-small cell lung cancer cells.  |  Rettig, M., et al. 2012. PLoS One. 7: e42012. PMID: 22848689
8. Crk II silencing down-regulates IGF-IR and inhibits migration and invasion of prostate cancer cells.  |  Dhupkar, P., et al. 2016. Biochem Biophys Rep. 8: 382-388. PMID: 28955980
9. Interaction in vitro of the product of the c-Crk-II proto-oncogene with the insulin-like growth factor I receptor.  |  Koval, AP., et al. 1998. Biochem J. 330 (Pt 2): 923-32. PMID: 9480911
10. Tyrosine phosphorylation and association of p130Cas and c-Crk II by ANG II in vascular smooth muscle cells.  |  Takahashi, T., et al. 1998. Am J Physiol. 274: H1059-65. PMID: 9575907

The preferred Crk II antibody is Crk II Antibody (B-4): sc-390132.