CRISP-1 CRISPR/Cas9 KO Plasmid (m): sc-419035
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CRISP-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CRISP-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRISP-1 CRISPR/Cas9 KO Plasmid (m) | sc-419035 | 20 µg | $397.00 |
Overview
Crisp1 encodes cysteine-rich secretory protein 1 (CRISP-1), a member of the CAP (cysteine-rich secretory proteins/antigen 5/pathogenesis-related 1) superfamily that is highly expressed in the male reproductive tract and secreted into the epididymal lumen. In mouse, CRISP-1 associates with sperm during epididymal maturation and contributes to gamete interactions, including regulation of sperm capacitation and participation in sperm–zona pellucida binding and fertilization-related events. At the cellular level, CRISP-1 is linked to secretory pathway biology and extracellular protein interactions that modulate membrane and signaling properties of spermatozoa. Dysregulation of Crisp1 is relevant to studies of male fertility phenotypes and reproductive tract function, and it is also used as a model for CAP-family protein roles in mucosal and immune-related contexts.
CRISP-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Crisp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Crisp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Crisp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CRISP-1 protein expression.
This CRISPR knockout system enables efficient generation of Crisp1-deficient cell models for investigation of CRISP-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Crisp1 exon(s) critical for CRISP-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Crisp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CRISP-1 CRISPR/Cas9 KO Plasmid (m) and CRISP-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Crisp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CRISP-1 HDR Plasmid (m) and CRISP-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Crisp1 homology arms to support homology-directed repair at defined Crisp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.