CRIK Antibody (C-5): sc-377449

CRIK Antibody (C-5) is a mouse monoclonal IgG1 antibody that detects CRIK in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CRIK, also known as Citron Rho-interacting kinase, is a vital 2,027 amino acid cytoplasmic protein that plays a crucial role in the regulation of cytokinesis and the development of the central nervous system. CRIK is characterized by a complex structure, which includes an AGC-kinase C-terminal domain, a CNH domain, a PH domain, a phorbol-ester/DAG-type zinc finger, and a protein kinase domain, all contributing to functional versatility. CRIK′s structural integrity is essential for interaction with other proteins, such as KIF14, which is necessary for proper localization to the central spindle and midbody during cell division. CRIK exists as four alternatively spliced isoforms, providing a layer of regulatory complexity that can influence various cellular processes. The gene encoding CRIK is located on chromosome 12q24.23, highlighting genetic significance in cellular functions. Anti-CRIK antibody (C-5) is an invaluable tool for researchers aiming to explore the intricate roles of CRIK in cellular dynamics and implications in developmental biology and disease.

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CRIK Antibody (C-5) References:

1. Proteomic analysis of mantle-cell lymphoma by protein microarray.  |  Ghobrial, IM., et al. 2005. Blood. 105: 3722-30. PMID: 15650054
2. Hepatitis C virus NS5A is a direct substrate of casein kinase I-alpha, a cellular kinase identified by inhibitor affinity chromatography using specific NS5A hyperphosphorylation inhibitors.  |  Quintavalle, M., et al. 2007. J Biol Chem. 282: 5536-44. PMID: 17166835
3. Biallelic Mutations in Citron Kinase Link Mitotic Cytokinesis to Human Primary Microcephaly.  |  Li, H., et al. 2016. Am J Hum Genet. 99: 501-10. PMID: 27453578
4. E2F-2 Promotes Nuclear Condensation and Enucleation of Terminally Differentiated Erythroblasts.  |  Swartz, KL., et al. 2017. Mol Cell Biol. 37: PMID: 27795297
5. Transcriptomic analysis of gene expression profiles of stomach carcinoma reveal abnormal expression of mitotic components.  |  Tong, H., et al. 2017. Life Sci. 170: 41-49. PMID: 27923621
6. Mutations of KIF14 cause primary microcephaly by impairing cytokinesis.  |  Moawia, A., et al. 2017. Ann Neurol. 82: 562-577. PMID: 28892560
7. Biallelic variants in KIF14 cause intellectual disability with microcephaly.  |  Makrythanasis, P., et al. 2018. Eur J Hum Genet. 26: 330-339. PMID: 29343805
8. Citron Rho-interacting kinase silencing causes cytokinesis failure and reduces tumor growth in multiple myeloma.  |  Sahin, I., et al. 2019. Blood Adv. 3: 995-1002. PMID: 30940634
9. Inhibitors of Rho kinases (ROCK) induce multiple mitotic defects and synthetic lethality in BRCA2-deficient cells.  |  Martino, J., et al. 2023. Elife. 12: PMID: 37073955
10. Citron rho-interacting kinase, a novel tissue-specific ser/thr kinase encompassing the Rho-Rac-binding protein Citron.  |  Di Cunto, F., et al. 1998. J Biol Chem. 273: 29706-11. PMID: 9792683

The preferred CRIK antibody is CRIK Antibody (E-6): sc-390437.