CREB3L4 Double Nickase Plasmid (h): sc-417626-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CREB3L4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CREB3L4 Double Nickase Plasmid (h) and CREB3L4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CREB3L4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CREB3L4 Antibody (D-8): sc-514052
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CREB3L4 Double Nickase Plasmid (h) | sc-417626-NIC | 20 µg | $410.00 | |||
CREB3L4 Double Nickase Plasmid (h2) | sc-417626-NIC-2 | 20 µg | $410.00 |
CREB3L4 (cAMP responsive element binding protein 3-like 4) is an ER membrane–tethered bZIP transcription factor that can be activated by regulated intramembrane proteolysis to modulate gene expression programs linked to secretory pathway homeostasis. It participates in cellular responses connected to ER stress signaling and unfolded protein response–related transcriptional networks, influencing differentiation and metabolic adaptation. CREB3L4 activity has been studied in hormone-responsive tissues and cancers, where altered expression or downstream transcriptional outputs can correlate with changes in proliferation, survival signaling, and invasive phenotypes. These properties make CREB3L4 a useful target for dissecting transcriptional control mechanisms that connect ER function with disease-associated cellular states.
CREB3L4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CREB3L4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CREB3L4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CREB3L4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CREB3L4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.