creatine kinase-M/B Antibody (CK-10): sc-51843

Creatine kinase-M/B Antibody (CK-10) is a mouse monoclonal IgG antibody that detects creatine kinase-M/B in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Creatine kinase-M/B plays a crucial role in cellular energy metabolism, particularly in muscle and brain tissues, where creatine kinase-M/B facilitates the transfer of phosphate groups to regenerate adenosine triphosphate (ATP) from creatine phosphate. This process is vital for maintaining energy homeostasis, especially during periods of high energy demand, such as muscle contraction and neuronal activity. Creatine kinase-M/B is predominantly located in the cytoplasm of muscle cells and neurons, where creatine kinase-M/B ensures a rapid supply of ATP, thereby supporting essential functions like muscle contraction and neurotransmission. Proper functioning of creatine kinase-M/B is critical, as deficiencies or dysregulation can lead to energy deficits, resulting in muscle weakness, neurological disorders, and other metabolic issues. Anti-creatine kinase-M/B antibody (CK-10) is an invaluable tool for researchers studying energy metabolism and related pathologies, providing insights into the dynamics of ATP production and utilization in human tissues.

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creatine kinase-M/B Antibody (CK-10) References:

1. Creatine kinase B-driven energy transfer in the brain is important for habituation and spatial learning behaviour, mossy fibre field size and determination of seizure susceptibility.  |  Jost, CR., et al. 2002. Eur J Neurosci. 15: 1692-706. PMID: 12059977
2. Creatine kinase B deficient neurons exhibit an increased fraction of motile mitochondria.  |  Kuiper, JW., et al. 2008. BMC Neurosci. 9: 73. PMID: 18662381
3. Knockdown of creatine kinase B inhibits ovarian cancer progression by decreasing glycolysis.  |  Li, XH., et al. 2013. Int J Biochem Cell Biol. 45: 979-86. PMID: 23416112
4. Submembranous recruitment of creatine kinase B supports formation of dynamic actin-based protrusions of macrophages and relies on its C-terminal flexible loop.  |  Venter, G., et al. 2015. Eur J Cell Biol. 94: 114-27. PMID: 25538032
5. Inositol hexakisphosphate kinase-2 determines cellular energy dynamics by regulating creatine kinase-B.  |  Nagpal, L., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 33547244
6. Creatine kinase B controls futile creatine cycling in thermogenic fat.  |  Rahbani, JF., et al. 2021. Nature. 590: 480-485. PMID: 33597756
7. Cytochalasin Q exerts anti-melanoma effect by inhibiting creatine kinase B.  |  Lu, Y., et al. 2022. Toxicol Appl Pharmacol. 441: 115971. PMID: 35276125
8. Ginkgolide B targets and inhibits creatine kinase B to regulate the CCT/TRiC-SK1 axis and exerts pro-angiogenic activity in middle cerebral artery occlusion mice.  |  Zhu, J., et al. 2022. Pharmacol Res. 180: 106240. PMID: 35513225
9. Creatine kinase B suppresses ferroptosis by phosphorylating GPX4 through a moonlighting function.  |  Wu, K., et al. 2023. Nat Cell Biol. 25: 714-725. PMID: 37156912
10. Cytoplasmic extrusion and the switch from creatine kinase B to M isoform are completed by the commencement of epididymal transport in human and stallion spermatozoa.  |  Huszar, G., et al. 1998. J Androl. 19: 11-20. PMID: 9537287

The preferred creatine kinase-B antibody is creatine kinase-B Antibody (B-9): sc-373686.