CRABP-I/II Antibody (F-9): sc-166897

CRABP-I/II Antibody (F-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects CRABP-I/II protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CRABP-I/II (F-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The cellular retinoic acid-binding proteins, CRABP-I and CRABP-II, play crucial roles in regulating retinoic acid (RA), a vital molecule involved in cell growth and differentiation across various tissues. CRABP-I functions primarily to inhibit RA activity by promoting expression of enzymes that metabolize RA, thereby facilitating RA degradation. In contrast, CRABP-II enhances RA′s effects by directly interacting with retinoic acid receptors (RAR), leading to formation of RAR-RA complexes that stimulate gene transcription associated with RA signaling. Both isoforms are expressed during embryonic development, with CRABP-I being widely distributed in adult tissues, while CRABP-II is predominantly found in tissues that produce significant amounts of RA, such as skin, uterus, ovary, and choroid plexus. Understanding distinct roles of these proteins is essential for elucidating contributions to developmental processes and potential implications in various diseases.

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CRABP-I/II Antibody (F-9) References:

1. Distinct roles for cellular retinoic acid-binding proteins I and II in regulating signaling by retinoic acid.  |  Dong, D., et al. 1999. J Biol Chem. 274: 23695-8. PMID: 10446126
2. Retinoid signalling and gene expression in neuroblastoma cells: RXR agonist and antagonist effects on CRABP-II and RARbeta expression.  |  Hewson, QC., et al. 2002. J Cell Biochem. 87: 284-91. PMID: 12397610
3. The level of CRABP-I expression influences the amounts and types of all-trans-retinoic acid metabolites in F9 teratocarcinoma stem cells.  |  Boylan, JF. and Gudas, LJ. 1992. J Biol Chem. 267: 21486-91. PMID: 1328234
4. Differential distribution patterns of CRABP I and CRABP II transcripts during mouse embryogenesis.  |  Ruberte, E., et al. 1992. Development. 115: 973-87. PMID: 1333403
5. Overexpression of cellular retinoic acid-binding protein type II (CRABP-II) and down-regulation of CRABP-I in psoriatic skin.  |  Siegenthaler, G., et al. 1992. Dermatology. 185: 251-6. PMID: 1335801
6. Differential expression of CRABP-II in fibroblasts derived from dermis and subcutaneous fat.  |  Van den Bogaerdt, AJ., et al. 2004. Biochem Biophys Res Commun. 315: 428-33. PMID: 14766225
7. Vitamin E activates CRABP-II gene expression in cultured human fibroblasts, role of protein kinase C.  |  Gimeno, A., et al. 2004. FEBS Lett. 569: 240-4. PMID: 15225641
8. Molecular cloning of cDNA encoding a second cellular retinoic acid-binding protein.  |  Giguère, V., et al. 1990. Proc Natl Acad Sci U S A. 87: 6233-7. PMID: 2166951
9. Molecular cloning and transcriptional mapping of the mouse cellular retinoic acid-binding protein gene.  |  Wei, LN., et al. 1990. DNA Cell Biol. 9: 471-8. PMID: 2171550
10. Retinoic acid induction of human cellular retinoic acid-binding protein-II gene transcription is mediated by retinoic acid receptor-retinoid X receptor heterodimers bound to one far upstream retinoic acid-responsive element with 5-base pair spacing.  |  Aström, A., et al. 1994. J Biol Chem. 269: 22334-9. PMID: 8071361
11. The cellular retinoic acid binding protein I is dispensable.  |  Gorry, P., et al. 1994. Proc Natl Acad Sci U S A. 91: 9032-6. PMID: 8090764
12. Localization of cellular retinoic acid-binding protein (CRABP) II and CRABP in developing rat testis.  |  Zheng, WL., et al. 1996. Endocrinology. 137: 5028-35. PMID: 8895377

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CRABP-I/II Antibody (F-9) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-CRABP-I/II Antibody (F-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CRABP-I/II Antibody (F-9) Specifications

Alternate Product Name: CRABP-I/II

Product Clone Name: F-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 26-130

Epitope Target: CRABP-I

Epitope Species: human

Product Reactivity/Detection: CRABP-I and CRABP-II

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for CRABP-I/II Antibody (F-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin