CPTII Antibody (G-5): sc-377294

CPTII Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects CPTII protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-CPTII antibody (G-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CPTII protein plays a crucial role in the mitochondrial β-oxidation of long-chain fatty acids, specifically functioning as a key enzyme that catalyzes the final step in the transport of acylcarnitines into the mitochondria for energy production. This process is vital for maintaining cellular energy homeostasis, particularly in tissues that rely heavily on fatty acid oxidation, such as the heart and skeletal muscle. Deficiencies in CPTII can lead to metabolic disorders, highlighting the importance of CPTII protein in both normal physiology and disease states. CPTII (G-5) antibody is an essential tool for researchers studying fatty acid metabolism, mitochondrial function, and related metabolic disorders, providing reliable detection and characterization of CPTII protein across various experimental applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CPTII Antibody (G-5) References:

1. Selective modulation of carnitine long-chain acyltransferase activities. Kinetics, inhibitors, and active sites of COT and CPT-II.  |  Ramsay, RR. and Gandour, RD. 1999. Adv Exp Med Biol. 466: 103-9. PMID: 10709633
2. Functional characterization of mammalian mitochondrial carnitine palmitoyltransferases I and II expressed in the yeast Pichia pastoris.  |  Woldegiorgis, G., et al. 2000. J Nutr. 130: 310S-314S. PMID: 10721894
3. A new nonsense mutation is associated with dysmorphism in lethal neonatal CPTII deficiency.  |  McGill, JS., et al. 2012. Clin Dysmorphol. 21: 179-181. PMID: 22652984
4. Inter-tissue and inter-species characteristics of the mitochondrial carnitine palmitoyltransferase enzyme system.  |  Woeltje, KF., et al. 1990. J Biol Chem. 265: 10714-9. PMID: 2355017
5. Regulation of ketogenesis and the renaissance of carnitine palmitoyltransferase.  |  McGarry, JD., et al. 1989. Diabetes Metab Rev. 5: 271-84. PMID: 2656156
6. Severe Hyperammonemic Encephalopathy Requiring Dialysis Aggravated by Prolonged Fasting and Intermittent High Fat Load in a Ramadan Fasting Month in a Patient with CPTII Homozygous Mutation.  |  Phowthongkum, P., et al. 2018. JIMD Rep. 41: 11-16. PMID: 29159461
7. Successful orthotopic heart transplantation in CPTII deficiency.  |  Arnold, GL., et al. 2021. Mol Genet Metab. 133: 182-184. PMID: 34020866
8. Human liver mitochondrial carnitine palmitoyltransferase I: characterization of its cDNA and chromosomal localization and partial analysis of the gene.  |  Britton, CH., et al. 1995. Proc Natl Acad Sci U S A. 92: 1984-8. PMID: 7892212
9. Isolation and characterization of cDNA and genomic clones encoding human muscle type carnitine palmitoyltransferase I.  |  Yamazaki, N., et al. 1996. Biochim Biophys Acta. 1307: 157-61. PMID: 8679700
10. Structural features of the gene encoding human muscle type carnitine palmitoyltransferase I.  |  Yamazaki, N., et al. 1997. FEBS Lett. 409: 401-6. PMID: 9224698
11. Functional studies of yeast-expressed human heart muscle carnitine palmitoyltransferase I.  |  Zhu, H., et al. 1997. Arch Biochem Biophys. 347: 53-61. PMID: 9344464
12. Characterization of carnitine acylcarnitine translocase system of heart mitochondria.  |  Pande, SV. and Parvin, R. 1976. J Biol Chem. 251: 6683-91. PMID: 977593

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CPTII Antibody (G-5) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-CPTII Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CPTII Antibody (G-5) Specifications

Alternate Product Name: Carnitine Palmitoyltransferase 2

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 51-350

Epitope Target: CPTII

Epitope Species: human

Product Reactivity/Detection: CPTII

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for CPTII Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin