CPSF6 Double Nickase Plasmid (h): sc-404919-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CPSF6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CPSF6 Double Nickase Plasmid (h) and CPSF6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CPSF6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CPSF6 Antibody (F-3): sc-376228
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CPSF6 Double Nickase Plasmid (h) | sc-404919-NIC | 20 µg | $410.00 | |||
CPSF6 Double Nickase Plasmid (h2) | sc-404919-NIC-2 | 20 µg | $410.00 |
CPSF6 (cleavage and polyadenylation specificity factor 6) is a core component of the CFIm complex that helps define 3′ end processing of pre-mRNAs by regulating poly(A) site choice and alternative polyadenylation. Through coupling of cleavage/polyadenylation with transcription and splicing, CPSF6 contributes to mRNA maturation, transcript stability, and gene expression programs that shape cell state. CPSF6 also interfaces with RNA-binding and nuclear export processes and has been implicated in host–pathogen interactions, including HIV-1 nuclear entry and integration site targeting. Dysregulation of 3′ end processing and alternative polyadenylation linked to CPSF6 function is associated with altered oncogenic signaling and disease-relevant transcriptome remodeling in diverse human cell types.
CPSF6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CPSF6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CPSF6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CPSF6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CPSF6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.