CPSF6 Antibody (F-3): sc-376228
- CPSF6 Antibody (F-3) is a mouse monoclonal IgG1 κ CPSF6 antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 167-225 mapping within an internal region of CPSF6 of human origin
- CPSF6 Antibody (F-3) is recommended for detection of CPSF6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine and bovine
- Anti-CPSF6 Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CPSF6 Antibody (F-3). This work is in progress.
CPSF6 Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects CPSF6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-CPSF6 antibody (F-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CPSF6, also known as CFIm68, plays a crucial role in the processing of 3′ ends of eukaryotic mRNAs, which involves endonucleolytic cleavage and polyadenylation. This process is essential for the stability and translation of mRNA, impacting gene expression and cellular function. CPSF6 is a key component of the cleavage and polyadenylation specificity factor (CPSF) complex, which is vital for the first step in pre-mRNA processing. Structurally, CPSF6 contains an N-terminal RNA recognition motif and a C-terminal RS-like domain, allowing CPSF6 to interact with other proteins such as SRp20, Tra-2β, and 9G8. CPSF6 localizes to paraspeckles and forms a heterodimer with NUDT21, contributing to the CFIm complex that is essential for mRNA processing. The presence of CPSF6 in only half of the heterodimer combinations highlights a unique role in the regulation of mRNA metabolism, making CPSF6 a significant target for research in gene expression and related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CPSF6 Antibody (F-3) References:
- A history of poly A sequences: from formation to factors to function. | Edmonds, M. 2002. Prog Nucleic Acid Res Mol Biol. 71: 285-389. PMID: 12102557
- Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein interactions, and subcellular localization. | Dettwiler, S., et al. 2004. J Biol Chem. 279: 35788-97. PMID: 15169763
- Entamoeba histolytica: comparative genomics of the pre-mRNA 3' end processing machinery. | López-Camarillo, C., et al. 2005. Exp Parasitol. 110: 184-90. PMID: 15955310
- An interaction between U2AF 65 and CF I(m) links the splicing and 3' end processing machineries. | Millevoi, S., et al. 2006. EMBO J. 25: 4854-64. PMID: 17024186
- Subnuclear localization and dynamics of the Pre-mRNA 3' end processing factor mammalian cleavage factor I 68-kDa subunit. | Cardinale, S., et al. 2007. Mol Biol Cell. 18: 1282-92. PMID: 17267687
- Pre-messenger RNA cleavage factor I (CFIm): potential role in alternative polyadenylation during spermatogenesis. | Sartini, BL., et al. 2008. Biol Reprod. 78: 472-82. PMID: 18032416
- The t(1;9)(p34;q34) and t(8;12)(p11;q15) fuse pre-mRNA processing proteins SFPQ (PSF) and CPSF6 to ABL and FGFR1. | Hidalgo-Curtis, C., et al. 2008. Genes Chromosomes Cancer. 47: 379-85. PMID: 18205209
- Sequence similarity between the 73-kilodalton protein of mammalian CPSF and a subunit of yeast polyadenylation factor I. | Jenny, A., et al. 1996. Science. 274: 1514-7. PMID: 8929409
- Characterization of a Drosophila homologue of the 160-kDa subunit of the cleavage and polyadenylation specificity factor CPSF. | Salinas, CA., et al. 1998. Mol Gen Genet. 257: 672-80. PMID: 9604891
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CPSF6 Antibody (F-3) | sc-376228 | 200 µg/ml | $322.00 | |||
CPSF6 Antibody (F-3) AC | sc-376228 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CPSF6 Antibody (F-3) HRP | sc-376228 HRP | 200 µg/ml | $322.00 | |||
CPSF6 Antibody (F-3) FITC | sc-376228 FITC | 200 µg/ml | $336.00 | |||
CPSF6 Antibody (F-3) PE | sc-376228 PE | 200 µg/ml | $349.00 | |||
CPSF6 Antibody (F-3) Alexa Fluor® 488 | sc-376228 AF488 | 200 µg/ml | $364.00 | |||
CPSF6 Antibody (F-3) Alexa Fluor® 546 | sc-376228 AF546 | 200 µg/ml | $364.00 | |||
CPSF6 Antibody (F-3) Alexa Fluor® 594 | sc-376228 AF594 | 200 µg/ml | $364.00 | |||
CPSF6 Antibody (F-3) Alexa Fluor® 647 | sc-376228 AF647 | 200 µg/ml | $364.00 | |||
CPSF6 Antibody (F-3) Alexa Fluor® 680 | sc-376228 AF680 | 200 µg/ml | $364.00 | |||
CPSF6 Antibody (F-3) Alexa Fluor® 790 | sc-376228 AF790 | 200 µg/ml | $364.00 |