cPLA2 Antibody (H-12): sc-376636

cPLA2 Antibody (H-12) is a mouse monoclonal IgG3 antibody that detects cytosolic phospholipase A2 (cPLA2) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). cPLA2 plays a crucial role in phospholipid hydrolysis, specifically targeting the sn-2 position to release arachidonic acid, a key precursor for synthesizing various eicosanoids, including prostaglandins and leukotrienes, which are significant mediators of inflammatory responses. Anti-cPLA2 antibody (H-12) enables detection in diverse cell types, such as platelets, macrophages, and monoblasts, where cPLA2 localizes in the cytosol for rapid mobilization and release of arachidonic acid in response to cellular stimuli, influencing numerous physiological and pathological processes, including inflammation, pain, and immune responses. cPLA2 (H-12) antibody serves as an essential tool for researchers investigating inflammation mechanisms and eicosanoid roles in various diseases, providing reliable detection and characterization of this vital enzyme in different biological contexts.

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cPLA2 Antibody (H-12) References:

1. BK Induces cPLA2 Expression via an Autocrine Loop Involving COX-2-Derived PGE2 in Rat Brain Astrocytes.  |  Lin, CC., et al. 2015. Mol Neurobiol. 51: 1103-15. PMID: 24915969
2. cPLA2 and desaturases underlie the tau hyperphosphorylation offset induced by BACE knock-down in neuronal primary cultures.  |  Villamil-Ortiz, JG. and Cardona-Gómez, GP. 2018. Biochim Biophys Acta Mol Basis Dis. 1864: 3696-3707. PMID: 30251689
3. A calcium-dependent phospholipase A2 (cPLA2) expression is regulated by MIG-6 during endometrial tumorigenesis.  |  Teasley, HE., et al. 2019. Biochem Biophys Res Commun. 511: 129-134. PMID: 30773264
4. cPLA2 activation contributes to lysosomal defects leading to impairment of autophagy after spinal cord injury.  |  Li, Y., et al. 2019. Cell Death Dis. 10: 531. PMID: 31296844
5. Tetrahydrocurcumin Downregulates MAPKs/cPLA2 Signaling and Attenuates Platelet Thromboxane A2 Generation, Granule Secretion, and Thrombus Growth.  |  Li, W., et al. 2022. Thromb Haemost. 122: 739-754. PMID: 34428833
6. Dapagliflozin Reduces Apoptosis of Diabetic Retina and Human Retinal Microvascular Endothelial Cells Through ERK1/2/cPLA2/AA/ROS Pathway Independent of Hypoglycemic.  |  Hu, Y., et al. 2022. Front Pharmacol. 13: 827896. PMID: 35281932
7. Altered hippocampal expression and function of cytosolic phospholipase A2 (cPLA2) in temporal lobe epilepsy (TLE).  |  Bera, A., et al. 2022. Neurol Res. 44: 748-753. PMID: 35285418
8. Involvement of p38 MAPK/cPLA2 and arachidonic acid metabolic pathway in Shengmai injection-induced pseudo-allergic reactions.  |  Wang, D., et al. 2023. J Ethnopharmacol. 309: 116357. PMID: 36906156
9. [Neuroprotective effect and mechanism of cPLA2 inhibitor increases autophagic flux on spinal cord injury].  |  Yan, WH., et al. 2023. Zhongguo Gu Shang. 36: 873-9. PMID: 37735081
10. YTHDF3-mediated FLCN/cPLA2 axis improves cardiac fibrosis via suppressing lysosomal function.  |  Zhang, Y., et al. 2025. Acta Pharmacol Sin.. PMID: 39806064

The preferred cPLA₂ antibody is cPLA₂ Antibody (4-4B-3C): sc-454.