CPE Double Nickase Plasmid (h): sc-404429-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CPE Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CPE Double Nickase Plasmid (h) and CPE Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CPE. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CPE Antibody (H-6): sc-393760
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CPE Double Nickase Plasmid (h) | sc-404429-NIC | 20 µg | $410.00 | |||
CPE Double Nickase Plasmid (h2) | sc-404429-NIC-2 | 20 µg | $410.00 |
Carboxypeptidase E (CPE) is a secretory pathway exopeptidase that removes C-terminal basic residues from prohormone and proneuropeptide intermediates, supporting the maturation of bioactive peptides in endocrine and neuroendocrine cells. Beyond its catalytic role, CPE participates in dense-core secretory granule trafficking and regulated secretion, linking peptide processing to vesicular sorting and stimulus-coupled release. Altered CPE expression or function has been associated with disrupted metabolic and neuroendocrine homeostasis, and has been studied in contexts such as obesity-related phenotypes, diabetes susceptibility, and neurological dysfunction. These features make CPE a useful node for dissecting peptide hormone biosynthesis, secretory granule biology, and signaling networks dependent on processed neuropeptides.
CPE Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CPE locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CPE. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CPE function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CPE-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.