Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
COX5b CRISPR/Cas9 KO Plasmid (h): sc-405159
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COX5b CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the COX5b genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: COX5b Antibody (C-5): sc-374416
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COX5b CRISPR/Cas9 KO Plasmid (h) | sc-405159 | 20 µg | $397.00 |
Overview
COX5B encodes cytochrome c oxidase subunit 5B (COX5b), a nuclear-encoded component of mitochondrial complex IV that supports electron transfer from cytochrome c to molecular oxygen and contributes to proton pumping across the inner mitochondrial membrane. By modulating oxidative phosphorylation efficiency, COX5b influences cellular ATP production, redox balance, and mitochondrial membrane potential, integrating with pathways that coordinate mitochondrial biogenesis and stress responses. Altered complex IV function and COX5B expression are frequently studied in contexts of mitochondrial dysfunction, including bioenergetic remodeling in neurodegeneration, cardiometabolic disorders, and tumor metabolism. COX5B is also used as a marker gene in investigations of respiratory chain assembly, reactive oxygen species signaling, and mitochondrial-nuclear cross-talk.
COX5b CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the COX5B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the COX5B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the COX5B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish COX5b protein expression.
This CRISPR knockout system enables efficient generation of COX5B-deficient cell models for investigation of COX5b signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting COX5B exon(s) critical for COX5b function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple COX5B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by COX5b CRISPR/Cas9 KO Plasmid (h) and COX5b CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the COX5B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by COX5b HDR Plasmid (h) and COX5b HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by COX5B homology arms to support homology-directed repair at defined COX5B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.