COX2 Antibody (D-5): sc-514489

COX2 Antibody (D-5) is a mouse monoclonal IgM antibody that detects COX2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-COX2 antibody (D-5) is available as the non-conjugated form. Cytochrome c oxidase subunit II (COX2), also known as MTCO2 or COII, is a crucial component of the oxidative phosphorylation complex IV, which plays a vital role in cellular respiration. Located in the mitochondrial inner membrane, COX2 is one of three subunits encoded by mitochondrial DNA that form the catalytic core of respiratory complex IV, alongside COX1 and COX3. This enzyme complex is responsible for the reduction of molecular oxygen to water, a process that releases energy used to pump protons across the mitochondrial inner membrane, creating an electrochemical gradient essential for ATP synthesis. Proper functioning of COX2 is critical, as defects can lead to impaired energy production and are associated with various pathologies, including tumor formation. Anti-COX2 antibody (D-5) effectively detects COX2 across multiple applications, making this reagent an invaluable tool for researchers studying mitochondrial function and related diseases.

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COX2 Antibody (D-5) References:

1. Cytochrome oxidase in health and disease.  |  Barrientos, A., et al. 2002. Gene. 286: 53-63. PMID: 11943460
2. Role of the conserved arginine pair in proton and electron transfer in cytochrome C oxidase.  |  Qian, J., et al. 2004. Biochemistry. 43: 5748-56. PMID: 15134449
3. Phosphorylation of Y845 on the epidermal growth factor receptor mediates binding to the mitochondrial protein cytochrome c oxidase subunit II.  |  Boerner, JL., et al. 2004. Mol Cell Biol. 24: 7059-71. PMID: 15282306
4. Analysis of cytochrome C oxidase subunits III and IV expression in developing rat brain.  |  Cannino, G., et al. 2004. Neuroscience. 128: 91-8. PMID: 15450356
5. Separation of mammalian cytochrome c oxidase into 13 polypeptides by a sodium dodecyl sulfate-gel electrophoretic procedure.  |  Kadenbach, B., et al. 1983. Anal Biochem. 129: 517-21. PMID: 6303162
6. Structure of cytochrome c oxidase.  |  Capaldi, RA., et al. 1983. Biochim Biophys Acta. 726: 135-48. PMID: 6307356

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-COX2 Antibody (D-5) has 72 citations in a variety of scientific publications.

Primary antibodies like Anti-COX2 Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

COX2 Antibody (D-5) Specifications

Alternate Product Name: COX2

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 169-192

Epitope Target: COX2

Epitope Species: human

Product Reactivity/Detection: COX2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 72 citations in various scientific publications

Available Conjugates/Forms for COX2 Antibody (D-5): P: Blocking Peptide