Cox-2 Antibody (H-3): sc-376861

Cox-2 Antibody (H-3) is a mouse monoclonal IgM kappa antibody specific for an epitope mapping between amino acids 17-55 near the N-terminus of Cyclooxygenase-2 (Cox-2) of rat origin; anti-Cox-2 antibody (H-3) reacts with mouse, rat, and human proteins and is suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), and immunohistochemistry. Cox-2, also known as Prostaglandin-Endoperoxide Synthase 2 (PTGS2), is an inducible enzyme that plays a crucial role in prostaglandin biosynthesis from arachidonic acid, mediating processes like inflammation, pain, and fever. Structurally, Cox-2 is a homodimeric enzyme with each subunit containing a heme prosthetic group essential for catalytic activity; Cox-2 possesses both cyclooxygenase and peroxidase active sites within a single polypeptide chain. The structural differences between Cox-2 and Cox-1 isoform, such as a larger substrate channel and a distinct hydrophobic pocket, allow for selective inhibition by certain nonsteroidal anti-inflammatory drugs (NSAIDs), minimizing gastrointestinal side effects associated with non-selective NSAIDs. Understanding Cox-2 structure is important for developing selective inhibitors that can effectively target inflammatory pathways without affecting physiological functions mediated by Cox-1. Cox-2 overexpression has been implicated in various pathological conditions, including chronic inflammation and tumor progression, making Cox-2 a valuable target for therapeutic intervention and research. Cox-2 (H-3) monoclonal antibody serves as an essential tool for researchers studying Cox-2 expression, structural biology, and disease mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cox-2 Antibody (H-3) has 179 citations in a variety of scientific publications.

Primary antibodies like Anti-Cox-2 Antibody (H-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cox-2 Antibody (H-3) Specifications

Alternate Product Name: Cox-2

Product Clone Name: H-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 17-55

Epitope Target: Cox-2

Epitope Species: rat

Product Reactivity/Detection: Cox-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 179 citations in various scientific publications

Available Conjugates/Forms for Cox-2 Antibody (H-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Cox-2 antibody is Cox-2 Antibody (29): sc-19999.