Coronin 1B Antibody (G-8): sc-271445
- Coronin 1B Antibody (G-8) is a mouse monoclonal IgG2b κ Coronin 1B antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 390-489 mapping at the C-terminus of Coronin 1B of human origin
- Coronin 1B Antibody (G-8) is recommended for detection of Coronin 1B of human origin by WB, IP, IF and ELISA
- Anti-Coronin 1B Antibody (G-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for Coronin 1B Antibody (G-8) for WB applications. This reagent is now offered in a bundle with Coronin 1B Antibody (G-8) (see ordering information below).
QUICK LINKS
Coronin 1B Antibody (G-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects Coronin 1B of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Coronin 1B antibody (G-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Coronin 1B plays a crucial role in regulating cell motility and cytoskeletal dynamics, primarily through interactions with the Arp2/3 complex, which is essential for actin polymerization and lamellipodia formation. Phosphorylation of Coronin 1B at Serine 2 by protein kinase C modulates leading edge dynamics of migrating cells, influencing fibroblast movement and cellular responses to environmental cues. Coronin 1B belongs to a family of coronin proteins sharing structural similarities, including WD repeat domains that facilitate protein-protein interactions, vital for processes like phagocytosis and intracellular trafficking. Understanding Coronin 1B function and regulation helps explain its role in cellular motility and potential implications in diseases characterized by altered cell movement, such as cancer metastasis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Coronin 1B Antibody (G-8) References:
- Coronin localizes to leading edges and is involved in cell spreading and lamellipodium extension in vertebrate cells. | Mishima, M. and Nishida, E. 1999. J Cell Sci. 112 (Pt 17): 2833-42. PMID: 10444378
- Oligomerization, F-actin interaction, and membrane association of the ubiquitous mammalian coronin 3 are mediated by its carboxyl terminus. | Spoerl, Z., et al. 2002. J Biol Chem. 277: 48858-67. PMID: 12377779
- Purification and functional characterization of the human N-CoR complex: the roles of HDAC3, TBL1 and TBLR1. | Yoon, HG., et al. 2003. EMBO J. 22: 1336-46. PMID: 12628926
- Coronin 7, the mammalian POD-1 homologue, localizes to the Golgi apparatus. | Rybakin, V., et al. 2004. FEBS Lett. 573: 161-7. PMID: 15327992
- Association of the leukocyte plasma membrane with the actin cytoskeleton through coiled coil-mediated trimeric coronin 1 molecules. | Gatfield, J., et al. 2005. Mol Biol Cell. 16: 2786-98. PMID: 15800061
- Coronin 3 and its role in murine brain morphogenesis. | Hasse, A., et al. 2005. Eur J Neurosci. 21: 1155-68. PMID: 15813925
- Coronin-1 function is required for phagosome formation. | Yan, M., et al. 2005. Mol Biol Cell. 16: 3077-87. PMID: 15829569
- Coronin proteins as multifunctional regulators of the cytoskeleton and membrane trafficking. | Rybakin, V. and Clemen, CS. 2005. Bioessays. 27: 625-32. PMID: 15892111
- Phosphorylation of coronin 1B by protein kinase C regulates interaction with Arp2/3 and cell motility. | Cai, L., et al. 2005. J Biol Chem. 280: 31913-23. PMID: 16027158
- F-actin binding is essential for coronin 1B function in vivo. | Cai, L., et al. 2007. J Cell Sci. 120: 1779-90. PMID: 17456547
- The Wilms' tumor suppressor Wt1 regulates Coronin 1B expression in the epicardium. | Hsu, WH., et al. 2013. Exp Cell Res. 319: 1365-81. PMID: 23562652
- Coronin 1B regulates S1P-induced human lung endothelial cell chemotaxis: role of PLD2, protein kinase C and Rac1 signal transduction. | Usatyuk, PV., et al. 2013. PLoS One. 8: e63007. PMID: 23667561
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Coronin 1B Antibody (G-8) | sc-271445 | 200 µg/ml | $322.00 | |||
Coronin 1B Antibody (G-8): m-IgG2b BP-HRP Bundle | sc-548701 | 200 µg Ab; 10 µg BP | $361.00 | |||
Coronin 1B Antibody (G-8) AC | sc-271445 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Coronin 1B Antibody (G-8) HRP | sc-271445 HRP | 200 µg/ml | $322.00 | |||
Coronin 1B Antibody (G-8) FITC | sc-271445 FITC | 200 µg/ml | $336.00 | |||
Coronin 1B Antibody (G-8) PE | sc-271445 PE | 200 µg/ml | $349.00 | |||
Coronin 1B Antibody (G-8) Alexa Fluor® 488 | sc-271445 AF488 | 200 µg/ml | $364.00 | |||
Coronin 1B Antibody (G-8) Alexa Fluor® 546 | sc-271445 AF546 | 200 µg/ml | $364.00 | |||
Coronin 1B Antibody (G-8) Alexa Fluor® 594 | sc-271445 AF594 | 200 µg/ml | $364.00 | |||
Coronin 1B Antibody (G-8) Alexa Fluor® 647 | sc-271445 AF647 | 200 µg/ml | $364.00 | |||
Coronin 1B Antibody (G-8) Alexa Fluor® 680 | sc-271445 AF680 | 200 µg/ml | $364.00 | |||
Coronin 1B Antibody (G-8) Alexa Fluor® 790 | sc-271445 AF790 | 200 µg/ml | $364.00 |