connexin 36 CRISPR Activation Plasmid (h): sc-402740-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- connexin 36 CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- connexin 36 CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by connexin 36 CRISPR Activation Plasmid (h) and connexin 36 CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the GJD2 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: connexin 36 Antibody (H-9): sc-398063
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
connexin 36 CRISPR Activation Plasmid (h) | sc-402740-ACT | 20 µg | $397.00 | |||
connexin 36 CRISPR Activation Plasmid (h2) | sc-402740-ACT-2 | 20 µg | $397.00 |
GJD2 encodes connexin 36 (Cx36), a gap junction protein that assembles into intercellular channels enabling direct electrical and metabolic coupling between adjacent cells. Cx36-mediated coupling supports synchronized firing and network oscillations, particularly in neuronal circuits, by permitting rapid ionic flux and small-molecule exchange across membranes. In the central nervous system and other excitable tissues, connexin 36 influences synaptic integration, signal propagation, and activity-dependent plasticity through regulation of gap junction assembly and gating. Altered GJD2/connexin 36 expression or coupling has been implicated in neurophysiological dysfunction and is studied in the context of disorders involving impaired neuronal synchrony.
connexin 36 CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous GJD2 expression without altering the underlying DNA sequence.
connexin 36 CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the GJD2 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the GJD2 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous connexin 36 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native GJD2 locus and enabling the study of connexin 36-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of connexin 36 pathway restoration in tumor cells with silenced or reduced GJD2 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.