connexin 26 Double Nickase Plasmid (h): sc-402132-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- connexin 26 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- connexin 26 Double Nickase Plasmid (h) and connexin 26 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GJB2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: connexin 26 Antibody (1C6): sc-293223
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
connexin 26 Double Nickase Plasmid (h) | sc-402132-NIC | 20 µg | $410.00 | |||
connexin 26 Double Nickase Plasmid (h2) | sc-402132-NIC-2 | 20 µg | $410.00 |
GJB2 encodes connexin 26, a gap junction channel subunit that oligomerizes into connexons to mediate direct intercellular exchange of ions and small metabolites. Connexin 26–containing gap junctions coordinate tissue homeostasis by supporting electrical and metabolic coupling, regulating epithelial barrier physiology, and shaping calcium and second-messenger signaling. In the inner ear and stratified epithelia, connexin 26 contributes to potassium ion recycling and cellular differentiation programs important for sensory and skin function. Disruption of GJB2 is strongly associated with hereditary hearing loss and syndromic ectodermal phenotypes, making it a key target for mechanistic studies of gap junction biology and tissue-specific signaling networks.
connexin 26 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GJB2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GJB2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GJB2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GJB2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.