Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
COMT Double Nickase Plasmid (h): sc-401823-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COMT Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- COMT Double Nickase Plasmid (h) and COMT Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting COMT. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: COMT Antibody (G-4): sc-137253
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COMT Double Nickase Plasmid (h) | sc-401823-NIC | 20 µg | $410.00 | |||
COMT Double Nickase Plasmid (h2) | sc-401823-NIC-2 | 20 µg | $410.00 |
Catechol-O-methyltransferase (COMT) is a cytosolic and membrane-associated methyltransferase that inactivates catechol substrates by transferring a methyl group from S-adenosyl-L-methionine to catecholamines and catechol estrogens. By regulating dopamine, norepinephrine, and epinephrine turnover, COMT influences neurotransmitter homeostasis and cellular responses to oxidative and metabolic stress. COMT activity interfaces with monoamine metabolism pathways alongside MAO-dependent degradation and impacts redox balance through catechol oxidation cycling. Genetic and expression variation in COMT has been studied in the context of neuropsychiatric phenotypes, pain sensitivity, and hormone-related biology, supporting its relevance as a mechanistic target in biomedical research.
COMT Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the COMT locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within COMT. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt COMT function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of COMT-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.