Complexin-1/2 Antibody (D-9): sc-365152

Complexin-1/2 Antibody (D-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects Complexin-1/2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-Complexin-1/2 antibody (D-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Complexin-1 and Complexin-2, also known as Synaphin-1 and Synaphin-2, are critical proteins that contain an alpha-helical middle domain of approximately 58 amino acids, which is essential for synaptic transmission. These proteins are predominantly expressed in presynaptic terminals of both inhibitory and excitatory neurons in the hippocampus, as well as in cytoplasmic pools during early developmental stages. Complexin-1 and Complexin-2 are vital for regulating neurotransmitter release, as these proteins facilitate SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) precomplex formation by binding to syntaxin through their alpha-helical domain. This interaction enables calcium-dependent release of neurotransmitters, as neurons lacking complexins show reduced transmitter release efficiency due to diminished calcium sensitivity in synaptic secretion. Additionally, Complexin-2 plays a role in long-term potentiation following tetanic stimulation, contributing to synaptic plasticity. A progressive loss of Complexin-2 has been observed in brains of mice with the Huntington disease mutation, suggesting that altered neurotransmitter release may contribute to motor, emotional, and cognitive dysfunctions associated with this disorder.

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Complexin-1/2 Antibody (D-9) References:

1. Selective interaction of complexin with the neuronal SNARE complex. Determination of the binding regions.  |  Pabst, S., et al. 2000. J Biol Chem. 275: 19808-18. PMID: 10777504
2. Hippocampal synaptic pathology in schizophrenia, bipolar disorder and major depression: a study of complexin mRNAs.  |  Eastwood, SL. and Harrison, PJ. 2000. Mol Psychiatry. 5: 425-32. PMID: 10889554
3. Involvement of complexin II in synaptic plasticity in the CA1 region of the hippocampus: the use of complexin II-lacking mice.  |  Huang, GZ., et al. 2000. Jpn J Pharmacol. 84: 179-87. PMID: 11128041
4. Progressive depletion of complexin II in a transgenic mouse model of Huntington's disease.  |  Morton, AJ. and Edwardson, JM. 2001. J Neurochem. 76: 166-72. PMID: 11145989
5. Complexins regulate a late step in Ca2+-dependent neurotransmitter release.  |  Reim, K., et al. 2001. Cell. 104: 71-81. PMID: 11163241
6. SNARE complex oligomerization by synaphin/complexin is essential for synaptic vesicle exocytosis.  |  Tokumaru, H., et al. 2001. Cell. 104: 421-32. PMID: 11239399
7. Structural organization of the human complexin 2 gene (CPLX2) and aspects of its functional activity.  |  Raevskaya, NM., et al. 2005. Gene. 359: 127-37. PMID: 16162394
8. Complexin-1 and synaptotagmin-1 compete for binding sites on membranes containing PtdInsP2.  |  Liang, Q., et al. 2022. Biophys J. 121: 3370-3380. PMID: 36016497
9. Analysis of tripartite Synaptotagmin-1-SNARE-complexin-1 complexes in solution.  |  Jaczynska, K., et al. 2023. FEBS Open Bio. 13: 26-50. PMID: 36305864
10. Complexin-1 regulated assembly of single neuronal SNARE complex revealed by single-molecule optical tweezers.  |  Hao, T., et al. 2023. Commun Biol. 6: 155. PMID: 36750663
11. Complexin-1 enhances ultrasound neurotransmission in the mammalian auditory pathway.  |  Liu, M., et al. 2024. Nat Genet. 56: 1503-1515. PMID: 38834904
12. Complexins: cytosolic proteins that regulate SNAP receptor function.  |  McMahon, HT., et al. 1995. Cell. 83: 111-9. PMID: 7553862

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Complexin-1/2 Antibody (D-9) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Complexin-1/2 Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Complexin-1/2 Antibody (D-9) Specifications

Alternate Product Name: Complexin-1/2

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-134

Epitope Target: Complexin-1

Epitope Species: human

Product Reactivity/Detection: Complexin-1 and Complexin-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Complexin-1/2 Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin