Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
COMMD1 CRISPR/Cas9 KO Plasmid (h): sc-404544
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COMMD1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the COMMD1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: COMMD1 Antibody (B-3): sc-518177
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COMMD1 CRISPR/Cas9 KO Plasmid (h) | sc-404544 | 20 µg | $397.00 |
Overview
COMMD1 (copper metabolism MURR1 domain-containing protein 1) is a multifunctional adaptor protein that regulates ubiquitin-dependent signaling and membrane trafficking, with prominent roles in copper homeostasis and intracellular protein turnover. It modulates NF-κB transcriptional activity by influencing the stability and nuclear dynamics of key pathway components, thereby impacting inflammatory and stress-response programs. COMMD1 also interfaces with endosomal sorting machinery and ion transport processes, contributing to epithelial and hepatic physiology. Altered COMMD1 function has been linked to dysregulated metal handling, oxidative stress susceptibility, and aberrant inflammatory signaling relevant to studies of liver biology, metabolic stress, and cancer-associated signaling networks.
COMMD1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the COMMD1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the COMMD1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the COMMD1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish COMMD1 protein expression.
This CRISPR knockout system enables efficient generation of COMMD1-deficient cell models for investigation of COMMD1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting COMMD1 exon(s) critical for COMMD1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple COMMD1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by COMMD1 CRISPR/Cas9 KO Plasmid (h) and COMMD1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the COMMD1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by COMMD1 HDR Plasmid (h) and COMMD1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by COMMD1 homology arms to support homology-directed repair at defined COMMD1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.