COL9A1 Antibody (H-7): sc-376969

COL9A1 Antibody (H-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects COL9A1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). COL9A1 (H-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Collagen Type IX protein, encoded by COL9A1 gene, plays a crucial role in the structural integrity of cartilage and the eye, as COL9A1 is a key component of the extracellular matrix. COL9A1 is particularly important due to its unique structure, which includes a large N-terminal globular domain that facilitates interactions with other collagen types, such as Collagen Type II. The formation of a heterotrimer with Collagen α2 Type IX and Collagen α3 Type IX enhances the stability and functionality of collagen fibrils, which are essential for maintaining the mechanical properties of cartilage. Additionally, COL9A1 gene is expressed in the human inner ear, and COL9A1 disruption has been linked to hearing loss in mice, highlighting the significance of Collagen Type IX in auditory function. Mutations in COL9A1 gene are associated with multiple epiphyseal dysplasia, a condition that affects bone growth and development in humans, further underscoring the importance of COL9A1 in skeletal health.

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COL9A1 Antibody (H-7) References:

1. A mutation in COL9A1 causes multiple epiphyseal dysplasia: further evidence for locus heterogeneity.  |  Czarny-Ratajczak, M., et al. 2001. Am J Hum Genet. 69: 969-80. PMID: 11565064
2. Regulation of human COL9A1 gene expression. Activation of the proximal promoter region by SOX9.  |  Zhang, P., et al. 2003. J Biol Chem. 278: 117-23. PMID: 12399468
3. Type IX collagen is crucial for normal hearing.  |  Asamura, K., et al. 2005. Neuroscience. 132: 493-500. PMID: 15802199
4. Trabecular bone deterioration in col9a1+/- mice associated with enlarged osteoclasts adhered to collagen IX-deficient bone.  |  Wang, CJ., et al. 2008. J Bone Miner Res. 23: 837-49. PMID: 18251701
5. The complete primary structure of two distinct forms of human alpha 1 (IX) collagen chains.  |  Muragaki, Y., et al. 1990. Eur J Biochem. 192: 703-8. PMID: 2209617
6. Structure of the glycosaminoglycan domain in the type IX collagen-proteoglycan.  |  McCormick, D., et al. 1987. Proc Natl Acad Sci U S A. 84: 4044-8. PMID: 3473493
7. Clinical Characteristics of Short-Stature Patients With Collagen Gene Mutation and the Therapeutic Response to rhGH.  |  Chen, M., et al. 2022. Front Endocrinol (Lausanne). 13: 820001. PMID: 35250876
8. Assessment of the COL9A1 Single Nucleotide Polymorphism with Severity of clubfoot in a paediatric population along with their biological mothers.  |  Raikwar, A., et al. 2022. J Clin Orthop Trauma. 35: 102064. PMID: 36420106
9. A thorough analysis of data on the correlation between COL9A1 polymorphisms and the susceptibility to congenital talipes equinovarus: a meta-analysis.  |  Golshan-Tafti, M., et al. 2024. J Orthop Surg Res. 19: 345. PMID: 38858754
10. Mice lacking alpha 1 (IX) collagen develop noninflammatory degenerative joint disease.  |  Fässler, R., et al. 1994. Proc Natl Acad Sci U S A. 91: 5070-4. PMID: 8197187
11. Physical and linkage mapping of the human and murine genes for the alpha 1 chain of type IX collagen (COL9A1).  |  Warman, ML., et al. 1993. Genomics. 17: 694-8. PMID: 8244386
12. Human COL9A1 and COL9A2 genes. Two genes of 90 and 15 kb code for similar polypeptides of the same collagen molecule.  |  Pihlajamaa, T., et al. 1998. Matrix Biol. 17: 237-41. PMID: 9707347

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-COL9A1 Antibody (H-7) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-COL9A1 Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

COL9A1 Antibody (H-7) Specifications

Alternate Product Name: COL9A1

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 532-612

Epitope Target: Collagen α1 Type IX

Epitope Species: human

Product Reactivity/Detection: Collagen α1 Type IX

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for COL9A1 Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin