?: sc-111465

CNTF (h): 293T Lysate is derived from the 293T cell line, a human embryonic kidney cell line modified to express the SV40 large T antigen, enhancing its transfection efficiency and protein expression capabilities. This lysate is enriched with human Ciliary Neurotrophic Factor (CNTF), a cytokine that supports the survival, differentiation, and maintenance of neurons. CNTF exerts its effects by binding to the CNTF receptor complex, which activates the JAK/STAT signaling pathway, leading to the transcription of genes involved in cell survival and differentiation. In research, CNTF (h): 293T Lysate is utilized to study the molecular mechanisms of neurotrophic support and cytokine signaling. Researchers employ this lysate in Western blotting to detect and quantify CNTF expression under various experimental conditions, providing insights into its regulation and functional roles. The lysate is also used in binding assays to examine the interaction between CNTF and its receptor, helping to explain the initial steps of signal transduction. Additionally, CNTF (h): 293T Lysate is valuable in functional assays to assess the impact of CNTF on neuronal cells, such as promoting neurite outgrowth and preventing apoptosis. Co-immunoprecipitation assays using this lysate can identify protein-protein interactions within the CNTF receptor complex and downstream signaling molecules, offering insights into the broader signaling networks influenced by CNTF. The use of CNTF (h): 293T Lysate allows for detailed investigations into the molecular and cellular mechanisms underlying neurotrophic support and cytokine signaling. This research contributes to a deeper understanding of how CNTF influences neuronal function and survival.