CNTF Double Nickase Plasmid (h): sc-402471-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CNTF Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CNTF Double Nickase Plasmid (h) and CNTF Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CNTF. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CNTF Antibody (A-11): sc-25286
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CNTF Double Nickase Plasmid (h) | sc-402471-NIC | 20 µg | $410.00 | |||
CNTF Double Nickase Plasmid (h2) | sc-402471-NIC-2 | 20 µg | $410.00 |
Ciliary neurotrophic factor (CNTF) is a secreted cytokine of the IL-6 family that supports neuronal survival and glial differentiation, and it is also active in metabolic tissues. CNTF signals through a tripartite receptor complex composed of CNTFRα with LIFR and gp130, engaging JAK/STAT—particularly STAT3—as well as MAPK/ERK and PI3K/AKT pathways to shape transcriptional programs involved in stress responses and cell fate decisions. In human systems, altered CNTF signaling has been studied in the context of neurodegeneration, neuroinflammation, and retinal cell maintenance, and as a modulator of energy balance and adipocyte biology. These functional links make CNTF a useful node for interrogating cytokine-driven trophic signaling and cross-talk between neural and peripheral tissues.
CNTF Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CNTF locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CNTF. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CNTF function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CNTF-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.