CNT1 Double Nickase Plasmid (h): sc-404790-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CNT1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CNT1 Double Nickase Plasmid (h) and CNT1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC28A1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CNT1 Antibody (G-7): sc-515874
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CNT1 Double Nickase Plasmid (h) | sc-404790-NIC | 20 µg | $410.00 | |||
CNT1 Double Nickase Plasmid (h2) | sc-404790-NIC-2 | 20 µg | $410.00 |
SLC28A1 encodes concentrative nucleoside transporter 1 (CNT1), a high-affinity, sodium-dependent transporter that mediates cellular uptake of pyrimidine nucleosides such as uridine and cytidine across the plasma membrane. By controlling nucleoside salvage, CNT1 influences nucleotide pool homeostasis and supports DNA/RNA synthesis, linking membrane transport to replication, repair, and cell-cycle progression. CNT1 activity intersects with broader metabolic programs that balance de novo nucleotide biosynthesis versus salvage, and altered expression has been associated with changes in epithelial differentiation and proliferative capacity in multiple tissue contexts. As a result, SLC28A1 is frequently studied in nucleoside transport biology, metabolic stress responses, and mechanisms that modulate sensitivity to nucleoside availability in disease-relevant models.
CNT1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC28A1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC28A1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC28A1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC28A1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.