CNOT7 Antibody (18W): sc-101009

CNOT7 Antibody (18W) is a mouse monoclonal IgG2a kappa light chain antibody that detects CNOT7 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CNOT7 antibody (18W) is available as the non-conjugated format. CNOT7, also known as CCR4-NOT transcription complex, subunit 7, CAF1 (CCR4-associated factor 1), hCAF-1, or BTG1-binding factor 1, is a crucial component of the CCR4-NOT complex, which is involved in both transcription regulation and mRNA degradation. CNOT7 is predominantly located in the nucleus, where CNOT7 plays a vital role in various cellular processes, including the regulation of nuclear hormone receptor activities. CNOT7 contains specific domains that facilitate interaction with other proteins, such as RXRβ, TOB1, TOB2, BTG1, BTG2, and BTG3, which are essential for function in gene expression and mRNA turnover. CNOT7 knockout mice exhibit sterility and increased bone mass, highlighting CNOT7′s important role in spermatogenesis and function as a suppressor of bone mass and bone morphogenetic protein actions in osteoblasts. These characteristics make CNOT7 a critical target for studies related to reproductive biology and bone metabolism, further emphasizing the value of CNOT7 monoclonal antibody (18W) in research applications.

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CNOT7 Antibody (18W) References:

1. Association of ANA, a member of the antiproliferative Tob family proteins, with a Caf1 component of the CCR4 transcriptional regulatory complex.  |  Yoshida, Y., et al. 2001. Jpn J Cancer Res. 92: 592-6. PMID: 11429045
2. Analysis of the transcription regulator, CNOT7, as a candidate chromosome 8 tumor suppressor gene in colorectal cancer.  |  Flanagan, J., et al. 2003. Int J Cancer. 106: 505-509. PMID: 12845644
3. Oligo-astheno-teratozoospermia in mice lacking Cnot7, a regulator of retinoid X receptor beta.  |  Nakamura, T., et al. 2004. Nat Genet. 36: 528-33. PMID: 15107851
4. CCR4-associated factor CAF1 is an essential factor for spermatogenesis.  |  Berthet, C., et al. 2004. Mol Cell Biol. 24: 5808-20. PMID: 15199137
5. Abnormal sperm morphology caused by defects in Sertoli cells of Cnot7 knockout mice.  |  Ogawa, T., et al. 2004. Arch Histol Cytol. 67: 307-14. PMID: 15700538
6. Expression analysis of LacZ gene placed in the locus of Cnot7 exhibits its activity in osteoblasts in vivo and in mineralized nodules in vitro.  |  Washio-Oikawa, K., et al. 2006. J Cell Biochem. 99: 538-44. PMID: 16639713
7. Cnot7-null mice exhibit high bone mass phenotype and modulation of BMP actions.  |  Washio-Oikawa, K., et al. 2007. J Bone Miner Res. 22: 1217-23. PMID: 17451368
8. Components of the CCR4-NOT complex function as nuclear hormone receptor coactivators via association with the NRC-interacting Factor NIF-1.  |  Garapaty, S., et al. 2008. J Biol Chem. 283: 6806-16. PMID: 18180299
9. DEAD-box helicase eIF4A2 inhibits CNOT7 deadenylation activity.  |  Meijer, HA., et al. 2019. Nucleic Acids Res. 47: 8224-8238. PMID: 31180491
10. CNOT7 modulates biological functions of ovarian cancer cells via AKT signaling pathway.  |  Yu, J., et al. 2021. Life Sci. 268: 118996. PMID: 33412213
11. CNOT7 Outcompetes Its Paralog CNOT8 for Integration into The CCR4-NOT Complex.  |  Stoney, PN., et al. 2022. J Mol Biol. 434: 167523. PMID: 35248544
12. CNOT7 regulates lipid deposition in nonalcoholic fatty liver disease.  |  Li, J., et al. 2024. Biochem Biophys Res Commun. 721: 150003. PMID: 38772212

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CNOT7 Antibody (18W) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-CNOT7 Antibody (18W) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CNOT7 Antibody (18W) Specifications

Alternate Product Name: CNOT7

Product Clone Name: 18W

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-286

Epitope Target: CNOT7

Epitope Species: human

Product Reactivity/Detection: CNOT7

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for CNOT7 Antibody (18W): N/A