CMV pp86 Antibody (12E2): sc-69835
- CMV pp86 Antibody (12E2) is a mouse monoclonal IgG1 κ, cited in 10 publications, provided at 200 µg/ml
- raised against recombinant partial length pp86 of CMV origin
- CMV pp86 Antibody (12E2) is recommended for detection of pp86 of CMV origin by WB, IP and IF
- Anti-CMV pp86 Antibody (12E2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CMV pp86 Antibody (12E2) for WB applications. These reagents are now offered in bundles with CMV pp86 Antibody (12E2) (see ordering information below).
CMV pp86 Antibody (12E2) is a mouse monoclonal IgG1 kappa light chain antibody that detects CMV pp86 protein of Cytomegalovirus (CMV) origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-CMV pp86 antibody (12E2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CMV pp86 protein, also known as UL122, IE2, or IE86, plays a crucial role during active CMV infection by interacting with other immediate early proteins, such as CMV pp72, to enhance HLA-G expression, a non-classical major histocompatibility complex class I molecule. This interaction is significant as HLA-G is involved in immune evasion, allowing the virus to persist in the host by inhibiting immune response activation. Additionally, CMV pp86 is essential for activating CMV immediate early promoter, which is stimulated by inflammatory cytokines like tumor necrosis factor alpha, interleukin 1 beta, and interleukin 4, thereby facilitating viral replication and pathogenesis. Understanding CMV pp86 function is vital for developing therapeutic strategies against CMV infections, particularly in immunocompromised patients who are at higher risk for severe disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CMV pp86 Antibody (12E2) References:
- Modulation of HLA-G antigens expression by human cytomegalovirus: specific induction in activated macrophages harboring human cytomegalovirus infection. | Onno, M., et al. 2000. J Immunol. 164: 6426-34. PMID: 10843698
- Antisense oligonucleotide ISIS 2922 targets IE-expression and prevents HCMV-IE-induced suppression of TSP-1 and TSP-2 expression. | Margraf, S., et al. 2001. Nucleosides Nucleotides Nucleic Acids. 20: 1425-8. PMID: 11563036
- Kinetics of HCMV immediate early mRNA expression in stably transfected fibroblasts. | Snaar, SP., et al. 2002. J Cell Sci. 115: 321-8. PMID: 11839784
- Comparative analysis of CD8+ T cell responses against human cytomegalovirus proteins pp65 and immediate early 1 shows similarities in precursor frequency, oligoclonality, and phenotype. | Khan, N., et al. 2002. J Infect Dis. 185: 1025-34. PMID: 11930311
- Comparison of hCMV immediate early and CaMV 35S promoters in both plant and human cells. | Vlasák, J., et al. 2003. J Biotechnol. 103: 197-202. PMID: 12890606
- Evaluation of a microtiter plate fluorescent-antibody assay for rapid detection of human cytomegalovirus infection. | Boppana, SB., et al. 1992. J Clin Microbiol. 30: 721-3. PMID: 1313050
- CD8(+) T-cell immunity to cytomegalovirus. | Moss, P. and Khan, N. 2004. Hum Immunol. 65: 456-64. PMID: 15172445
- Simultaneous generation of cytomegalovirus-specific CD8+ and CD4+ T lymphocytes by use of dendritic cells comodified with pp65 mRNA and pp65 protein. | Carlsson, B., et al. 2005. J Infect Dis. 192: 1912-20. PMID: 16267762
- Comparison of HCMV IE and EF-1 promoters for the stable expression of beta-subunit of hexosaminidase in CHO cell lines. | Sinici, I., et al. 2006. Biochem Genet. 44: 173-80. PMID: 16645900
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CMV pp86 Antibody (12E2) | sc-69835 | 200 µg/ml | $316.00 | |||
CMV pp86 Antibody (12E2): m-IgG Fc BP-HRP Bundle | sc-526986 | 200 µg Ab; 10 µg BP | $354.00 | |||
CMV pp86 Antibody (12E2): m-IgG1 BP-HRP Bundle | sc-532359 | 200 µg Ab; 20 µg BP | $354.00 | |||
CMV pp86 Antibody (12E2) AC | sc-69835 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CMV pp86 Antibody (12E2) HRP | sc-69835 HRP | 200 µg/ml | $316.00 | |||
CMV pp86 Antibody (12E2) FITC | sc-69835 FITC | 200 µg/ml | $330.00 | |||
CMV pp86 Antibody (12E2) PE | sc-69835 PE | 200 µg/ml | $343.00 | |||
CMV pp86 Antibody (12E2) Alexa Fluor® 488 | sc-69835 AF488 | 200 µg/ml | $357.00 | |||
CMV pp86 Antibody (12E2) Alexa Fluor® 546 | sc-69835 AF546 | 200 µg/ml | $357.00 | |||
CMV pp86 Antibody (12E2) Alexa Fluor® 594 | sc-69835 AF594 | 200 µg/ml | $357.00 | |||
CMV pp86 Antibody (12E2) Alexa Fluor® 647 | sc-69835 AF647 | 200 µg/ml | $357.00 | |||
CMV pp86 Antibody (12E2) Alexa Fluor® 680 | sc-69835 AF680 | 200 µg/ml | $357.00 | |||
CMV pp86 Antibody (12E2) Alexa Fluor® 790 | sc-69835 AF790 | 200 µg/ml | $357.00 |