CMPK Antibody (H-2): sc-376153

CMPK Antibody (H-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects CMPK protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CMPK Antibody (H-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CMPK protein, also known as cytidine monophosphate kinase, UMP-CMP kinase, and deoxycytidylate kinase, is a crucial enzyme composed of 196 amino acids that catalyzes the transfer of a phosphate group from ATP to UMP, CMP, and dCMP, resulting in the production of ADP and the corresponding nucleoside diphosphate. This enzymatic activity is vital for cellular nucleic acid synthesis, as CMPK facilitates the activation of nucleoside monophosphate kinases, which are essential for the pharmacological activation of therapeutic nucleosides and nucleotide analogs. Primarily localized in the cytoplasm, CMPK also plays a significant role in the activation of pyrimidine analogs, which are clinically important in the development of anti-cancer and anti-viral drugs. Notably, CMP is the preferred substrate for CMPK, followed by UMP and dCMP, underscoring CMPK′s specificity and importance in nucleotide metabolism.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CMPK Antibody (H-2) References:

1. Phosphorylation of deoxycytidine analog monophosphates by UMP-CMP kinase: molecular characterization of the human enzyme.  |  Van Rompay, AR., et al. 1999. Mol Pharmacol. 56: 562-9. PMID: 10462544
2. Phosphorylation of nucleosides and nucleoside analogs by mammalian nucleoside monophosphate kinases.  |  Van Rompay, AR., et al. 2000. Pharmacol Ther. 87: 189-98. PMID: 11008000
3. Characterization of human UMP-CMP kinase enzymatic activity and 5' untranslated region.  |  Pearman, AT., et al. 2001. Life Sci. 69: 2361-70. PMID: 11681623
4. Characterization of human UMP/CMP kinase and its phosphorylation of D- and L-form deoxycytidine analogue monophosphates.  |  Liou, JY., et al. 2002. Cancer Res. 62: 1624-31. PMID: 11912132
5. Reaction of human UMP-CMP kinase with natural and analog substrates.  |  Pasti, C., et al. 2003. Eur J Biochem. 270: 1784-90. PMID: 12694191
6. Substrate-induced conformational changes in human UMP/CMP kinase.  |  Segura-Peña, D., et al. 2004. J Biol Chem. 279: 33882-9. PMID: 15163660
7. Phosphorylation of Cytidine, Deoxycytidine, and Their Analog Monophosphates by Human UMP/CMP Kinase Is Differentially Regulated by ATP and Magnesium.  |  Hsu, CH., et al. 2005. Mol Pharmacol. 67: 806-14. PMID: 15550676
8. Uridine monophosphate kinase: A new genetic polymorphism with possible clinical implications.  |  Giblett, ER., et al. 1974. Am J Hum Genet. 26: 627-35. PMID: 4138532

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CMPK Antibody (H-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-CMPK Antibody (H-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CMPK Antibody (H-2) Specifications

Alternate Product Name: CMPK

Product Clone Name: H-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 92-196

Epitope Target: CMPK

Epitope Species: human

Product Reactivity/Detection: CMPK

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: porcine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for CMPK Antibody (H-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin