Clusterin Double Nickase Plasmid (m): sc-419695-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Clusterin Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Clusterin Double Nickase Plasmid (m) and Clusterin Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Clu. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Clusterin-α Antibody (A-11): sc-166831
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Clusterin Double Nickase Plasmid (m) | sc-419695-NIC | 20 µg | $410.00 | |||
Clusterin Double Nickase Plasmid (m2) | sc-419695-NIC-2 | 20 µg | $410.00 |
Mouse Clu encodes clusterin, a secreted and intracellular glycoprotein that functions as an extracellular chaperone and modulator of protein quality control under stress. Clusterin participates in lipid transport and complement regulation, shaping membrane homeostasis and inflammatory signaling, and is linked to pathways governing apoptosis, oxidative stress responses, and proteostasis. In the nervous system and peripheral tissues, altered CLU expression has been associated with protein aggregation phenotypes, neuroinflammation, and age-related tissue remodeling. These properties make Clu a useful target for dissecting stress-adaptive networks, extracellular proteome dynamics, and complement-associated cellular responses in mouse models.
Clusterin Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Clu locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Clu. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Clu function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Clu-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.