Clusterin Antibody (Hs-3): sc-51579

Clusterin Antibody (Hs-3) is a mouse monoclonal IgG1 antibody engineered to recognize Clusterin, a multifunctional secretory glycoprotein, by targeting an epitope raised against human sperm. Clusterin monoclonal antibody (Hs-3) is recommended for use in western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) applications, ensuring reliable detection of Clusterin in human samples. Clusterin, also known as apolipoprotein J (ApoJ), is a heterodimeric protein composed of α and β subunits linked by disulfide bonds, resulting from the post-translational cleavage of a 449 amino acid precursor at the internal bond between Arg227 and Ser228. The α subunit (ApoJβ) spans residues 228-449, while the β subunit (ApoJα) encompasses residues 23-227, together forming a stable and functional complex. This intricate structure is crucial for Clusterin′s diverse roles in various physiological processes, including immune regulation, cell adhesion, lipid transportation, tissue remodeling, membrane recycling, and cell-cell interactions. The heterodimeric nature of Clusterin allows interaction with a wide range of proteins and cellular components, facilitating involvement in maintaining cellular homeostasis and protecting cells from stress-induced damage. Additionally, structural integrity of Clusterin is essential for function as a complement lysis inhibitor, preventing unwanted complement activation and protecting cells from immune-mediated lysis. The ability of Clusterin to form stable complexes through disulfide bonds also underpins a role in binding and neutralizing misfolded proteins, thereby contributing to cellular protection mechanisms. Aberrations in Clusterin structure and expression are linked to various pathological conditions, including cancer, where overexpression is often associated with tumor progression and metastasis, and neurodegenerative diseases such as Alzheimer′s disease, where Clusterin influences β-amyloid aggregation and neuritic toxicity. Clusterin (Hs-3) antibody is recommended for studies of structural conformation and interactions within different cellular environments, providing valuable insights into functional mechanisms and role in disease pathogenesis.

The preferred Clusterin-α antibody is Clusterin-α Antibody (B-5): sc-5289.