CLS1 Double Nickase Plasmid (h): sc-407799-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CLS1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CLS1 Double Nickase Plasmid (h) and CLS1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CRLS1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CLS1 Antibody (E-8): sc-514986
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLS1 Double Nickase Plasmid (h) | sc-407799-NIC | 20 µg | $410.00 | |||
CLS1 Double Nickase Plasmid (h2) | sc-407799-NIC-2 | 20 µg | $410.00 |
CRLS1 encodes cardiolipin synthase 1 (CLS1), a mitochondrial inner membrane enzyme that catalyzes the final step of cardiolipin biosynthesis from phosphatidylglycerol and CDP-diacylglycerol. Cardiolipin is a signature phospholipid required for organization and stability of oxidative phosphorylation complexes, maintenance of cristae architecture, and efficient mitochondrial ATP production. Through its role in mitochondrial lipid remodeling and respiratory chain function, CLS1 influences apoptosis signaling, mitophagy, and cellular responses to metabolic stress. Altered cardiolipin homeostasis and impaired mitochondrial bioenergetics linked to CRLS1 dysfunction are relevant to studies of neuromuscular and cardiometabolic pathophysiology, as well as broader mitochondrial disease mechanisms.
CLS1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CRLS1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CRLS1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CRLS1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CRLS1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.