ClpP Antibody (B-12) HRP: sc-271284 HRP. Direct western blot analysis of ClpP expression in K-562 (A), Hep G2 (C), HeLa (D), Sol8 (E) and RAW 264.7 (F) whole cell lysates and mouse heart tissue extract (B).
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ClpP Antibody (B-12) HRP: sc-271284 HRP. Direct western blot analysis of ClpP expression in K-562 (A), Hep G2 (C), HeLa (D), Sol8 (E) and RAW 264.7 (F) whole cell lysates and mouse heart tissue extract (B).
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
ClpP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ClpP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ClpP Antibody (B-12): sc-271284