CLP36 Antibody (B-9): sc-393084

CLP36 Antibody (B-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects CLP36 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CLP36 (B-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CLP36, also known as PDLIM1, CLIM1, or Elfin, is a 329 amino acid cytoplasmic protein that plays a crucial role in organizing the cytoskeleton by associating with actin stress fibers. CLP36 expression is notably high in skeletal muscle and heart tissues, while lower levels are found in the colon, small intestine, spleen, lung, placenta, kidney, liver, thymus, and pancreas. CLP36 functions as an adaptor protein, facilitating target protein recruitment to the cytoskeleton, thereby influencing cellular structure and signaling. CLP36 interacts with Clik1, a kinase, and recruits Clik1 to α-actinin-1, enhancing Clik1′s association with actin stress fibers. This interaction maintains cytoskeletal network integrity and ensures proper cellular function. CLP36 contains one PDZ domain and one LIM zinc-binding domain, essential for protein-protein binding capabilities. Human CLP36 shares 88% sequence similarity with its rat counterpart, indicating a conserved function across species, which underscores CLP36′s significance in various biological processes.

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CLP36 Antibody (B-9) References:

1. CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4.  |  Vallenius, T., et al. 2000. J Biol Chem. 275: 11100-5. PMID: 10753915
2. Interaction of hCLIM1, an enigma family protein, with alpha-actinin 2.  |  Kotaka, M., et al. 2000. J Cell Biochem. 78: 558-65. PMID: 10861853
3. Human CLP36, a PDZ-domain and LIM-domain protein, binds to alpha-actinin-1 and associates with actin filaments and stress fibers in activated platelets and endothelial cells.  |  Bauer, K., et al. 2000. Blood. 96: 4236-45. PMID: 11110697
4. Clik1: a novel kinase targeted to actin stress fibers by the CLP-36 PDZ-LIM protein.  |  Vallenius, T. and Mäkelä, TP. 2002. J Cell Sci. 115: 2067-73. PMID: 11973348
5. Photodynamic treatment (ALA-PDT) suppresses the expression of the oncogenic Bcr-Abl kinase and affects the cytoskeleton organization in K562 cells.  |  Pluskalová, M., et al. 2006. J Photochem Photobiol B. 83: 205-12. PMID: 16495075
6. Expression of the actin stress fiber-associated protein CLP36 in the human placenta.  |  Miehe, U., et al. 2006. Histochem Cell Biol. 126: 465-71. PMID: 16609848
7. Spliced isoforms of LIM-domain-binding protein (CLIM/NLI/Ldb) lacking the LIM-interaction domain.  |  Tran, YH., et al. 2006. J Biochem. 140: 105-19. PMID: 16815859
8. Calcium activation of the LMO4 transcription complex and its role in the patterning of thalamocortical connections.  |  Kashani, AH., et al. 2006. J Neurosci. 26: 8398-408. PMID: 16899735
9. Plasma membrane Ca(2+) -ATPase associates with CLP36, alpha-actinin and actin in human platelets.  |  Bozulic, LD., et al. 2007. Thromb Haemost. 97: 587-97. PMID: 17393022

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CLP36 Antibody (B-9) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-CLP36 Antibody (B-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CLP36 Antibody (B-9) Specifications

Alternate Product Name: CLP36

Product Clone Name: B-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 84-123

Epitope Target: CLP36

Epitope Species: human

Product Reactivity/Detection: CLP36

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for CLP36 Antibody (B-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin