Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Clock Double Nickase Plasmid (m): sc-419693-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Clock Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Clock Double Nickase Plasmid (m) and Clock Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Clock. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Clock Antibody (C-8): sc-271603
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Clock Double Nickase Plasmid (m) | sc-419693-NIC | 20 µg | $410.00 | |||
Clock Double Nickase Plasmid (m2) | sc-419693-NIC-2 | 20 µg | $410.00 |
Mouse Clock encodes a basic helix–loop–helix PAS transcription factor that heterodimerizes with ARNTL/BMAL1 to drive core circadian gene expression via E-box elements. CLOCK–BMAL1 activity coordinates feedback regulation through PER and CRY proteins and couples rhythmic transcription to chromatin remodeling, metabolic control, and cell-cycle timing. Through crosstalk with pathways such as cAMP/CREB signaling, glucocorticoid signaling, and nuclear receptor networks, Clock influences energy homeostasis and stress responses. Dysregulated circadian function involving CLOCK has been linked in research settings to sleep–wake phenotypes, metabolic syndrome-related traits, inflammation, and cancer-associated transcriptional programs.
Clock Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Clock locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Clock. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Clock function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Clock-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.