CLIP Antibody (cerCLIP.1): sc-12725

CLIP Antibody (cerCLIP.1) is a mouse monoclonal IgG1 kappa light chain antibody that detects CLIP in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). CLIP (cerCLIP.1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CLIP plays a crucial role in the immune response by acting as a residual fragment of the invariant chain that remains in the peptide-binding groove of major histocompatibility complex (MHC) class II molecules after the invariant chains are degraded. This function is vital as CLIP allows for the proper loading of antigenic peptides onto MHC class II molecules, which are essential for T cell recognition and activation. The exchange of CLIP for processed peptides is facilitated by the non-classical human leukocyte antigen HLA-DM, which not only removes CLIP but also ensures that MHC class II molecules are loaded with the appropriate peptides for effective immune presentation. Anti-CLIP antibody (cerCLIP.1) exhibits strong reactivity with surface class II-CLIP complexes, making CLIP (cerCLIP.1) monoclonal antibody an invaluable tool for identifying HLA class II-positive cells, cells with impaired HLA-DM activity, and tumor cells that have evaded detection by CD4-positive T cells.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CLIP Antibody (cerCLIP.1) References:

1. Nonclassical MHC class II molecules.  |  Alfonso, C. and Karlsson, L. 2000. Annu Rev Immunol. 18: 113-42. PMID: 10837054
2. HLA-DR molecules from an antigen-processing mutant cell line are associated with invariant chain peptides.  |  Riberdy, JM., et al. 1992. Nature. 360: 474-7. PMID: 1448172
3. CD74: an emerging opportunity as a therapeutic target in cancer and autoimmune disease.  |  Borghese, F. and Clanchy, FI. 2011. Expert Opin Ther Targets. 15: 237-51. PMID: 21208136
4. CD74 is a novel transcription regulator.  |  Gil-Yarom, N., et al. 2017. Proc Natl Acad Sci U S A. 114: 562-567. PMID: 28031488
5. Role of MIF1/MIF2/CD74 interactions in bladder cancer.  |  Woolbright, BL., et al. 2023. J Pathol. 259: 46-55. PMID: 36214539
6. CD74 Promotes a Pro-Inflammatory Tumor Microenvironment by Inducing S100A8 and S100A9 Secretion in Pancreatic Cancer.  |  Hong, WC., et al. 2023. Int J Mol Sci. 24: PMID: 37629174
7. The complete sequence of the mRNA for the HLA-DR-associated invariant chain reveals a polypeptide with an unusual transmembrane polarity.  |  Strubin, M., et al. 1984. EMBO J. 3: 869-72. PMID: 6586420
8. HLA-DM induces CLIP dissociation from MHC class II alpha beta dimers and facilitates peptide loading.  |  Denzin, LK. and Cresswell, P. 1995. Cell. 82: 155-65. PMID: 7606781
9. Transport and intracellular distribution of MHC class II molecules and associated invariant chain in normal and antigen-processing mutant cell lines.  |  Riberdy, JM., et al. 1994. J Cell Biol. 125: 1225-37. PMID: 8207055
10. Out damned CLIP! Out, I say!  |  Roche, PA. 1996. Science. 274: 526-7. PMID: 8928005
11. Negative regulation by HLA-DO of MHC class II-restricted antigen processing.  |  Denzin, LK., et al. 1997. Science. 278: 106-9. PMID: 9311912
12. Structure of a trimeric domain of the MHC class II-associated chaperonin and targeting protein Ii.  |  Jasanoff, A., et al. 1998. EMBO J. 17: 6812-8. PMID: 9843486

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CLIP Antibody (cerCLIP.1) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-CLIP Antibody (cerCLIP.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CLIP Antibody (cerCLIP.1) Specifications

Alternate Product Name: CLIP

Product Clone Name: cerCLIP.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 81-104

Epitope Target: CD74

Epitope Species: human

Product Reactivity/Detection: CLIP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for CLIP Antibody (cerCLIP.1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD74 antibody is CD74 Antibody (LN-2): sc-6262.